rologie i - European Congress of Virology

5 th European Congress of Virologywas found to undergo an irreversible molecular change following pre acidification.Our findings suggest that the gradual decrease of pH and thehigh K+ conditions in endosomes is required to prime the IAV virion forefficient uncoating following fusion at pH 5.0 in the late endosomes.REF 513Complete genome sequences of elephant endotheliotropic herpesviruses1A and 1B determined directly from fatal casesGavin WILKIE 1 , Andrew DAVISON 1 , Mick WATSON 2 , Karen KERR 1 ,Stephanie SANDERSON 3 , Tim BOUTS 4 , Falko STEINBACH 5 , AkbarDASTJERDI 51 MRC–University of Glasgow Centre for Virus Research, Glasgow, UNI-TED KINGDOM; 2 ARK Genomics, The Roslin Institute and Royal (Dick)School of Veterinary Studies, University of Edinburgh, Edinburgh, UNI-TED KINGDOM; 3 Chester Zoo, Chester, UNITED KINGDOM; 4 ZSLWhipsnade Zoo, Dunstable, UNITED KINGDOM; 5 Animal Health andVeterinary Laboratories Agency Weybridge, Addlestone, UNITED KING-DOMA highly lethal hemorrhagic disease associated with infection by elephantendotheliotropic herpesvirus (EEHV) poses a severe threat to Asian elephanthusbandry. We have used high throughput methods to sequencethe genomes of the two genotypes that are involved in most fatalities,namely EEHV1A and EEHV1B (species Elephantid herpesvirus 1, genusProboscivirus, subfamily Betaherpesvirinae, family Herpesviridae). Thesequences were determined from postmortem tissue samples, despite thedata containing tiny proportions of viral reads among reads from a host forwhich the genome sequence was not available. The EEHV1A genome is180,421 bp in size and consists of a unique sequence (174,601 bp) flankedby a terminal direct repeat (2,910 bp). The genome contains 116 predictedprotein coding genes, of which six are fragmented, and seven paralogousgene families are present. The EEHV1B genome is very similar to that ofEEHV1A in structure, size, and gene layout. Half of the EEHV1A geneslack orthologs in other members of subfamily Betaherpesvirinae, such ashuman cytomegalovirus (genus Cytomegalovirus) and human herpesvirus6A (genus Roseolovirus). Notable among these are 23 genes encodingtype 3 membrane proteins containing seven transmembrane domains (the7TM family) and seven genes encoding related type 2 membrane proteins(the EE50 family). The availability of the genome sequences will facilitatefuture research on the epidemiology, pathogenesis, diagnosis, andtreatment of EEHV associated disease.REF 514Investigaton of BDV, PPR and BTV infections in sheep in KyrgyzstanOrhan YAPICI 1 , Orhan YAPICI 1 , Oya BULUT 2 , Oguzhan AVCI 2 ,Mehmet KALE 3 , Mambetali TURSUMBETOV 4 , Sibel YAVRU 2 , AtillaSIMSEK 2 , Kudaybergen ABDIKERIMOV 41 University of Kyrgzstan Turkey Manas, Faculty of Veterinary Medicine,Department of Virology, Bishkek, KYRGZSTAN; 2 University of Selcuk,Faculty of Veterinary Medicine, Department of Virology, Konya, TURKEY;3 University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine, Departmentof Virology, Burdur, TURKEY; 4 Institute of Veterinary Research,Bishkek, KYRGZSTANThe aim of this study is to define seroprevalence of Border Disease Virus(BDV), Peste De Petits Ruminants (PPR) and Blue tongue Virus (BTV)infections in sheep. Blood serum samples were collected from Issyk Kul(144), Naryn (208), Talas (189) and Çuy (114) in Kyrgzstan. Serumsamples (655) were analyzed for presence of antibodies against BDV, PPR,and BTV by commercially available enzyme linked immunosorbent assays(ELISA). The tests were performed as per the manufacturer’s instructions.Seropositivity to BDV, PPR, and BTV were found to be 7.32%, 35.11%,and 36.94%, respectively. Abortions of sheep are the cause of considerableeconomic losses for the farmers. These infectious viral agents are easilyspreading among animals. Abortions among small ruminants in Kyrgzstanshould be attributed to mixed infections. Furthermore these infectionspathogenes are should be further investigated. Etiological agents of viralabortion in sheep needs further investigation. Preventing diseases is themost efficient and cost effective way of managing disease. After the determinationof the situation of causative viral agents, eradication control andmonitoring programs should be prepared.Keywords: BDV, BTV, ELISA, PPR, sheepREF 515The Respiratory Virus Network an initiative to collect and providedata on respiratory virus diseases via internetOrtwin ADAMS 1 , Rolf KAISER 2 , Barbara GÄRTNER 3 , BenediktWEISSBRICH 41 Institute for Virology, Düsseldorf, GERMANY; 2 Institute for Virology,Cologne, GERMANY; 3 Institute for Medical Microbiology and HospitalHygiene, Homburg, GERMANY; 4 Institute for Virology, Würzburg,GERMANYThe Respiratory Virus Network (RespiVir) started 2009 from an initiativeof the section “Clinical Virology” of the Gesellschaft für Virologie(GN). Meanwhile the network consists of more than 30 laborato¬riesfrom Austria, Switzerland and Germany. The online accessible databasecollects data from positive and negative results from different respiratoryviruses, detection methods and age and gender of hospitalized patients.Frequencies and localization of the different virus species are vi¬siblefor the participants and in the near future also for the public. In additionthe results are reported with comments to the participants in monthlyreports. Specialized centers perform (sub)typing or sequence analysis forcertain viruses. The database contains more than 250000 test results from27000 patients. This allows to monitor more precisely than in the pastthe yearly RSV activity, which allows a better guided start and stop ofthe RSV prophylaxis with Palavizumab (Synagis) in premature babies. Asecond example was the detection of high activity with HMPV during theperiod of the H1N1 new variant in 2009/2010. Rhinoviruses have peaksin spring and autumn, but can be detected throughout the year. We seeRespiVir as a tool that allows to collect the data from meanwhile routinelyperformed diagnostics and provide an added value from the sum of thedata by addressing the question, which viruses are cir¬culating currently,are there regional differences, etc. The structure of this network allowsa virtual biobank for the participants. For the future it is also planned toserve as a platform for bacterial respiratory infections like B. pertussis,M. pneumoniae, C. pneumoniae as well as for collecting data of viral andbacterial gastroenteritis.REF 516Respiratory viruses in hospitalised adults in Bern, Switzerland duringthe 2012/2013 seasonAlexander LUETHI, Maria Teresa BARBANI, Samuel ZUERCHER,Jacqueline STEINLIN, Sarah WASMER, Meri GORGIEVSKIVirology, Institute for Infectious Diseases, University of Bern, Bern, SWIT-ZERLANDBackground: Respiratory viruses are a significant cause of morbidityand mortality especially in immunocompromised patients. A sensitive andspecific assay is required to provide optimal clinical care and reduce therisk of nosocomial infections. We evaluated the novel Argene Multi Well(MWS) r gene real time PCR assay to detect respiratory viruses fromadult patient samples. Methods: Respiratory specimens (431 upper/119lower respiratory tract) from adult patients with respiratory infection wereprospectively collected and tested from October 2012 to April 2013 byVirologie, Vol 17, supplément 2, septembre 2013S263