rologie i - European Congress of Virology

5 th European Congress of Virologyother VP4, VP6 and VP7 sequences of porcine, bovine and human RV Cstrains available in GenBank. Subsequently, the phylogenetic and molecularevolutionary analysis was conducted using MEGA version 4. Thestudy was supported by the Ministry of Agriculture of the Czech Republic(Grant No. QH81061) and the Ministry of Education, Youth and Sportsof the Czech Republic (AdmireVet; Grant No. CZ.1.05/2.1.00/01.0006,ED0006/01/01).REF 302A Serological Investigation of Rift Valley Fever (RVF) Infection inCamel (Camelus dromedarius), Goitred gazella (Gazella subgutturosasubgutturosa) and Domestic Anatolian water buffaloes (Bubalusbubalis Linneaus)Sibel YAVRU 3 , Sibel GUR 1 , Mehmet KALE 2 , Orhan YAPICI 6 , NuriMAMAK 4 , Nural EROL 51 University of Afyon Kocatepe, Faculty of Veterinary Medicine, Departmentof Virology, Afyon, TURKEY; 2 University of Mehmet Akif Ersoy,Faculty of Veterinary Medicine, Department of Virology, Burdur, TUR-KEY; 3 University of Selcuk, Faculty of Veterinary Medicine, Departmentof Virology, Konya, TURKEY; 4 University of Mehmet Akif Ersoy, Faculty ofVeterinary Medicine, Department of Internal Medicine, Burdur, TURKEY;5 University of Adnan Menderes, Faculty of Veterinary Medicine, Departmentof Virology, Aydin, TURKEY; 6 University of Kyrgyzstan TurkeyManas, Faculty of Veterinary Medicine, Department of Virology, Bishkek,KYRGYZSTANRift Valley Fever (RVF) which is an acute Arboviral infection characterizedzoonotic is a member of the genus Phlebovirus, in the familyBunyaviridae. Since first determination of the disease in 1915, many studieshave been done. But there is little data about the infection in ourcountry. This study was investigated the serological of RVFV infectionwhich collected blood serum samples in camel, goitred gazella, domesticAnatolian water buffaloes by the Indirect RVFV ELISA (IDVet, France).Camel samples (72 pcs) in the different farms province of Aydin, gazellasamples (82 pcs) at Ceylanpinar State Farm were collected in 2005. Anatolianwater buffaloes samples in majority of small farms between 1999 and2001 were obtained from various provinces. These provinces are Afyon(168 pcs),Amasya (80 pcs), Samsun (69 pcs), Ankara (35 pcs), Sivas (21pcs), Tokat (19 pcs), Konya (10 pcs) and Elazig (8 pcs). As a result of thetest sample of 82 goitred gazella found to be seronegative for RVF, whilethe one of camel samples (1/71, 1.3%), was positive. While DomesticAnatolian water buffaloes negative in all the samples obtained from Sivas,Tokat, Konya and Elazig, Amasya, 12 (12/80, 15%), Ankara, 5 (5/35,14.2%), Samsun, 8 (8/69, 11.5%) and Afyon, 10 (10/168, 5.9%) sampleswere detected seroposiitive. A total of 35 water buffaloes samples weredetermined seropositive for RVFV.Key words: Buffalo, Camel, Gazella, Rift Valley Fever, SerologyREF 303First detection of Schmallenberg Virus Infections in SloveniaJoze GROM, Ivan TOPLAK, Vasilij COCIANCICH, DanijelaRIHTARIC, Tomislav PALLERToplak Ivan, Grom Jože, Cociancich Vasilij, Rihtaric Danijela, TomislavPaller University of Ljubljana, Veterinary faculty, NVI, Gerbiceva 60, 1000Ljubljana, SLOVENIAIn November 2011, Germany reported the first occurrence of novel Orthobunyavirus,named Schmallenberg virus (SBV), later the same virusemerged in number of European countries. The first clinical case of SBVinfections, confirmed by laboratory diagnosis of SBV in Slovenia, wasidentified in January 9th 2013 from a herd of sheep, but to the end ofMarch 2013 additional 12 bovine herds were identified SBV positive byreal time RT PCR. To identify the time of first introduction of new virusinfection on territory of Slovenia retrospective analysis of 42 cattle samplescollected from June to October 2012 were screening for SBV antibodiesusing ELISA test. First SBV antibody positive blood samples were identifiedfrom sample collected in August 29th 2012. High prevalence (82.8%)of SBV antibodies in cattle was detected from 87 randomly selected cattlesamples collected between January and February 2013. The detectionof virus and high seroprevalence on our territory means, that a new viraldisease has already spread to majority of our farms and that it may resultin increased losses due to abortions in pregnant animals and losses in neonatallambs, calves and kids. Slovenia is the first country of Balkan areawhere disease was confirmed with high prevalence and this new virus willbe probably detected also in other Balkan countries soon.REF 304Epidemiology of Bluetongue Infection in Northeast and SoutheastAnatoliaTaner KARAOGLU 1 , Irfan OZGUNLUK 2 , Yakup YILDIRIM 3 , CigdemOGUZOGLU 1 , Seval Bilge DAGALP 1 , Aykut OZKUL 1 , FerayALKAN 1 , Yilmaz AKCA 1 , Ibrahim BURGU 11 Ankara University, Faculty of Veterinary Medicine, Ankara, TURKEY;2 Harran University, Faculty of Veterinary Medicine, S.Urfa, TURKEY;3 Kafkas University, Faculty of Veterinary Medicine, Kars, TURKEYBluetongue is an important disease of ruminants and is caused by an arthropodborne Orbivirus, Reoviridae. The aim of this study was to establish awarning system to enable the early detection of BT virus serotype invasionsin northeast and southeast Anatolia through the deployment of ‘sentinelherds’ formed of seronegative individuals. According to serological monitoringdata, two sentinel herds were constituted: one from 114 seronegativecattle from the herd I in the northeast Anatolian region; and the other form101 seronegative cattle from the herd II in the southeast Anatolian region.All the cattle from the two sentinel herds were sampled 11 times. None ofthe 114 individuals from herd I showed seroconversion during any samplingperiod. However, seroconversion was observed in 32 cattle from theherd II at various sampling dates. The cross virus neutralization test performedagainst BT 4, BT 9, BT 16 on the blood sera showed seroconversionin 32 individuals, of which 28 had specific antibody against BT 4, BT 9and BT 16, 3 had them against BT 9 and BT 16, and 1 had them againstonly BT 9. The results showed that the antibody titers against BT 9 weremuch higher, so that these antibodies had low cross reaction with BT 4and BT 16. While antibody titers against BT 4 and BT 16 varied betweenundiluted samples to 1/80, antibody titers against BT 9 were detected between1/40 and 1/1280. The data had been obtained showed that BT 9 wasdetected as the dominant circulating virus type in the area.REF 305Seroprevalence of five arboviruses in sentinel cattle as part of nationalsurveillance in Korea, 2009 2012Yeon Hee KIM 1,2 , Jae Ku OEM 1 , Koung Ki LEE 1 , Seong Hee KIM 1 ,Myoung Heon LEE 1 , Se Chang PARK 21 Animal and Plant Quarantine Agency, Anyang, REPUBLIC OF KOREA;2 College of Veterinary Medicine and Research Institute for VeterinaryScience, Seoul, REPUBLIC OF KOREAFrom 2009 2012, national arbovirus surveillance was conducted on sentinelcattle, fewer than 1 year to investigate the possible circulationarboviruses in South Korea. We investigated the presence of antibodies toAkabane virus, Aino virus, Chuzan virus, Bovine ephemeral fever (BEF)virus, Ibaraki virus using serum neutralization test in 1,000 blood samplescollected every year. In 2009, seropositive rates for each virus were lessthan 13.3%, whereas the individual seropositive rates for each virus were40.2% for Akabane virus, 33.2% for Aino virus, 29.1% for Chuzan virus,7.5% for Ibaraki virus, 2.9% for BEF virus in 2010. The seropositiveVirologie, Vol 17, supplément 2, septembre 2013S205