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7th World Congress on Mummy Studies University of San Diego San ...

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e-hydrati<strong>on</strong>. soluti<strong>on</strong>s. with. skin. tissue. samples. from. different.<br />

mummy.types..Results.indicate.a.high.variability.<strong>of</strong>.the.methods.<br />

in.questi<strong>on</strong>.in.terms.<strong>of</strong>.re-hydrati<strong>on</strong>.efficiency,.preservati<strong>on</strong>.<strong>of</strong>.tissue.details.and.staining.characteristics..Mekota,.A.M.,.Vermehren,.M..(2005).Determinati<strong>on</strong>.<strong>of</strong>.optimal.rehydrati<strong>on</strong>,.fixati<strong>on</strong>.and.staining.methods.for.histological.and.immunohistochemical.analysis.<strong>of</strong>.<br />

mummified. s<strong>of</strong>t. tissues,. Biotechnique and Histochemistry 80(1),.<br />

7-.13.<br />

187 Molecular Investigati<strong>on</strong>s <strong>of</strong> Microbial Communities in Mummified<br />

Human Remains, FRANK MAIXNER 1 *, THOMAS<br />

RATTEI 2 , GUADELUPE PINAR 3 , KATJA STERFLINGER-<br />

GLEIXNER 3 , DARIO. PIOMBINO-MASCALI 1 , GIOVANNA<br />

CIPOLLINI 1 , and ALBERT zINK 1 . ( 1 EURAC. -. Institute. for.<br />

Mummies. and. the. Iceman,.Viale. Druso. 1,. 39100. Bolzano,. Italy;.<br />

2 Department. <strong>of</strong>. Computati<strong>on</strong>al. Systems. Biology,. <strong>University</strong>. <strong>of</strong>.<br />

Vienna,.Austria;. 3 Institute.<strong>of</strong>.Applied.Microbiology,.<strong>University</strong>.<strong>of</strong>.<br />

Nati<strong>on</strong>al. Resources. and.Applied. Life. Sciences,. Vienna,.Austria;.<br />

frank.maixner@eurac.edu).<br />

Mummified. human. remains. can. be. regarded. as. an. independent.<br />

“ecosystem“. in. which,. <strong>on</strong>. the. <strong>on</strong>e. hand,. highly. specialized.<br />

microorganism.thrive,.some.<strong>of</strong>.which.are.involved.in.degradati<strong>on</strong>.<br />

processes..On.the.other.hand,.traces.<strong>of</strong>.ancient.DNA.in.these.specimens.could.provide.detailed.insight.into.the.past.microbiome.<strong>of</strong>.this.mummy.c<strong>on</strong>sisting.<strong>of</strong>.beneficial.and.potentially.pathogenic.bacteria..<br />

Until. recently,. the. vast. majority. <strong>of</strong>. complex. microbial. communities.were.largely.underestimated.due.to.major.technical,.time,.<br />

and.financial.c<strong>on</strong>straints..Therefore,.our.knowledge.<strong>of</strong>.mummified.<br />

bodies.as.“ecosystems”.is.limited..However,.advancements.in.highthroughput.<br />

next-generati<strong>on</strong>. sequencing. technology. have. yielded.<br />

powerful.new.tools.in.terms.<strong>of</strong>.cost.effectiveness,.time.saving.and.<br />

data.recovery..“In-depth”.sequencing.<strong>of</strong>.phylogenetic.marker.genes.<br />

will.shed.light.into.the.spectacular.microbial.diversity.in.various.<br />

habitats.in.and.<strong>on</strong>.mummies.<br />

188 State-<strong>of</strong>-the-Art Ancient <strong>Mummy</strong> Research: Coprolites, KARL<br />

J. REINHARD. (School. <strong>of</strong>. Natural. Resources,. Hardin. Hall. 719,.<br />

<strong>University</strong>. <strong>of</strong>. Nebraska,. Lincoln,. NE. 68583-0987;. kreinhard1@<br />

mac.com).<br />

Coprolites.are.a.source.<strong>of</strong>.a.diversity.<strong>of</strong>.data.regarding.infecti<strong>on</strong>,.medicine,.diet,.and.envir<strong>on</strong>ment.<strong>of</strong>.the.decedent..New.methods.are.introduced.yearly.for.each.<strong>of</strong>.these.topics..Highlighted.in.<br />

this. presentati<strong>on</strong>. are. several. issues.. Refined. parasite. diagnosis. is.<br />

<strong>of</strong>fered.by.molecular.and.chemical.methods..The.analysis.<strong>of</strong>.starch.<br />

represents.the.latest.revoluti<strong>on</strong>.in.pale<strong>on</strong>utriti<strong>on</strong>.and.is.immediately.<br />

applicable. to. mummy. studies.. Starch. grains. not. <strong>on</strong>ly. show. what.<br />

starch. sources. were. eaten,. but. also. the. manner. <strong>of</strong>. preparati<strong>on</strong>. <strong>of</strong>.<br />

specific. foods.. Diatom. studies. <strong>of</strong>. coprolites. are. <strong>on</strong>. the. horiz<strong>on</strong>..<br />

Diatoms.reveal.different.aquatic.envir<strong>on</strong>ments.exploited.by.ancient.<br />

peoples..By.applying.aeropalynology.data.regarding.seas<strong>on</strong>.variati<strong>on</strong>s.in.natural.pollen.rain,.it.is.possible.to.determine.in.what.m<strong>on</strong>th.<br />

<strong>of</strong>.the.year.an.individual.died.<br />

When.ecological.and.parasitological.data.are.combined,.patterns.<strong>of</strong>.climate.variati<strong>on</strong>,.diet,.and.infecti<strong>on</strong>.can.be.discerned..For.<br />

example, El Niño.events.at.the.coast.<strong>of</strong>.Chile.caused.variati<strong>on</strong>.in.<br />

Chinchorro.exploitati<strong>on</strong>.<strong>of</strong>.fish.species..This.in.turn.affected.prevalence.<strong>of</strong>.tapeworm.infecti<strong>on</strong>.<br />

Taph<strong>on</strong>omy. is. a. c<strong>on</strong>cern..The. preservati<strong>on</strong>. <strong>of</strong>. remains. from.<br />

mummy.coprolites.is.variable..By.analysis.<strong>of</strong>.insects,.mites,.and.the.<br />

ecology.<strong>of</strong>.preservati<strong>on</strong>,.<strong>on</strong>e.can.assess.the.preservati<strong>on</strong>.potential.<br />

ABSTRACTS.–.Symposia<br />

<strong>of</strong>. the. micr<strong>of</strong>ossils. from. coprolites..This. will. allow. <strong>on</strong>e. to. interpret.whether.the.absence.<strong>of</strong>.a.class.<strong>of</strong>.micr<strong>of</strong>ossils.is.due.to.poor.preservati<strong>on</strong>.c<strong>on</strong>diti<strong>on</strong>s..In.c<strong>on</strong>clusi<strong>on</strong>,.a.holistic.approach.to.coprolite.analysis.results.in.the.placement.<strong>of</strong>.pathological.c<strong>on</strong>diti<strong>on</strong>s.in.<br />

behavior.and.envir<strong>on</strong>mental.c<strong>on</strong>texts.<br />

189 Preservati<strong>on</strong> <strong>of</strong> Human Mummified Remains,. ALBERT<br />

zINK 1 *, MARCO SAMADELLI 1 , DARIO PIOMBINO-MAS-<br />

CALI 1 , and EDUARD EGARTER VIGL 2 .( 1 EURAC.-.Institute.<br />

for.Mummies.and.the.Iceman,.Viale.Druso.1,.39100.Bolzano,.Italy;.<br />

2 Department.<strong>of</strong>.Pathological.Anatomy.and.Histology,.General.Hospital.Bolzano,.Italy;.albert.zink@eurac.edu).The.preservati<strong>on</strong>.<strong>of</strong>.human.mummified.remains.<strong>of</strong>ten.represents.a.major.problem,.both.in.the.original.find.sites.as.well.as.in.<br />

sec<strong>on</strong>dary.depositories,.such.as.collecti<strong>on</strong>s.or.museums..Changes.<br />

<strong>of</strong>.the.microclimate.surrounding.mummy.burials.that.can.be.caused.<br />

by.excavati<strong>on</strong>.or.building.activities,.tourism.exploitati<strong>on</strong>.or.natural.<br />

influences. <strong>of</strong>ten. lead. to. a. decline. <strong>of</strong>. the. natural. preservati<strong>on</strong>.<br />

c<strong>on</strong>diti<strong>on</strong>s..In.particular,.the.increase.<strong>of</strong>.humidity.and.insufficient.<br />

ventilati<strong>on</strong>.enhance.the.growth.and.spread.<strong>of</strong>.microorganisms.that.<br />

could.result.in.major.damage.to.mummified.remains..The.storage.<strong>of</strong>.<br />

mummies.in.museums.or.collecti<strong>on</strong>s.faces.the.specific.challenge.<strong>of</strong>.<br />

recreating.the.natural.climatic.c<strong>on</strong>diti<strong>on</strong>s..This.<strong>of</strong>ten.proves.problematic,.as.the.optimal.preservati<strong>on</strong>.c<strong>on</strong>diti<strong>on</strong>s.for.mummies.from.<br />

different.geographic.areas.are.not.always.fully.understood,.and.also.<br />

due.to.the.high.costs.required.for.maintaining.and.displaying.the.<br />

human.remains.<br />

We.present.examples.<strong>of</strong>.different.mummy.find.sites,.such.as.<br />

in.Egypt.and.Sicily,.where.the.natural.preservati<strong>on</strong>.c<strong>on</strong>diti<strong>on</strong>s.have.<br />

changed.and.how.this.has.influenced.the.natural.storage.<strong>of</strong>.these.<br />

mummies.. Moreover,. different. soluti<strong>on</strong>s. for. the. c<strong>on</strong>servati<strong>on</strong>. <strong>of</strong>.<br />

mummies.in.museums.will.be.discussed..As.an.example,.the.Tyrolean.Iceman.is.stored.in.a.specially.designed.cooling.chamber.that.emulates.the.cold.and.humid.envir<strong>on</strong>ment.in.which.he.was.naturally.preserved.for.more.than.5000.years..Modern.showcases.allow.the.use.<strong>of</strong>.nitrogen.and.a.precise.c<strong>on</strong>trol.<strong>of</strong>.temperature.and.humidity..<br />

In. this. c<strong>on</strong>text,.possible. soluti<strong>on</strong>s. for. more. efficient.and. less.<br />

expensive.preservati<strong>on</strong>.systems.will.be.c<strong>on</strong>sidered.<br />

190 Dental <strong>Studies</strong>,.ROGER SEILER* and FRANK J. RÜHLI.<br />

(Centre.for.Evoluti<strong>on</strong>ary.Medicine,.<strong>University</strong>.<strong>of</strong>.Zürich,.Switzerland;.rogerseiler@bluewin.ch).<br />

For.many.years,.mummies.have.been.systematically.examined,.<br />

e.g..in.Switzerland..We.are.currently.completing.the.inventory.<strong>of</strong>.<br />

Swiss.mummies.with.a.special.emphasis.<strong>on</strong>.oral.findings..When.it.is.<br />

interpret.mummy.oral.pathologies.in.more.detail..These.pathologies.<br />

are.dental.wear.such.as.abrasi<strong>on</strong>,.attriti<strong>on</strong>,.erosi<strong>on</strong>,.and.the.combinati<strong>on</strong>.there.<strong>of</strong>;.caries,.period<strong>on</strong>tal.destructi<strong>on</strong>.and.apical.lesi<strong>on</strong>s.<strong>of</strong>.<br />

the.alveolar.b<strong>on</strong>e..In.additi<strong>on</strong>,.possible.postmortem.changes.in.the.<br />

dentiti<strong>on</strong>.relative.to.the.mummificati<strong>on</strong>.process.may.be.found..The.<br />

use.<strong>of</strong>.3D-rec<strong>on</strong>structi<strong>on</strong>s.al<strong>on</strong>e.has.in.many.cases.limited.informative.value..Therefore,.axial.images.and.2D-panoramic.reformatted.<br />

images.are.necessary.in.order.to.obtain.additi<strong>on</strong>al.informati<strong>on</strong>.for.<br />

the.diagnosis.<strong>of</strong>.caries.and.period<strong>on</strong>tal.destructi<strong>on</strong>.in.mummies..We.<br />

are.also.trying.to.estimate.the.clinical.relevance.<strong>of</strong>.these.pathological.findings.in.mummies.in.general.<br />

103

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