7th World Congress on Mummy Studies University of San Diego San ...

ABSTRACTS.–.Contributed.Oral.Papers
AAASPD
CELL and MOLECULAR BIOLOGY SECTION
Tuesday, starting at 9:20 a.m. in KIPJ Room I
297 Biochemical and Biophysical Study of Functional Characteristics
of Hemoglobin Components from Caspian Sea Sturgeons
(Acipenser. persicus and Acipenser. stellatus) Blood, SHOHREH
ARIAEENEJAD 1 *, SHAHLA JAMILI 1,2 , MEHRAN HABIBI-
REzAEI 3 , MOHAMMAD REzA FATEMI 1 , NAJMEH POUR-
SASAN 4 , and ALIA MOOSAVI-MOVAHEDI 4 ( 1 Marine.Biology.
Department,.Science.and.Research.Branch,.Iua,.Pobox:.14515-775,.
Tehran,. Iran;. 2 Iranian. Fisheries. Research. Organization. of. Iran;.
3 School.of.Biology,.College.of.Science,.University.Of.Tehran,.Tehran,.Iran;.
4 Institute.of.Biochemistry.and.Biophysics,.University.Of.
Tehran,.Tehran,.Iran;.Ariaee.sh@srbiau.ac.ir).
Hemoglobin. (Hb). multiplicity. enables. fishes. to. adapt. themselves.with.different.ecological.and.environmental.conditions..In.
the. current. study,. the. Hbs. of. two. Iranian. Sturgeon. species. have.
been.investigated..After.extraction.of.Hb.from.blood,.the.isoelectric.focusing.study.has.shown.that.both.species.have.multiple.Hbs.with.different.isoelectric.points.and.the.dominant.Hb.can.be.identified.from.other.Hb.components..The.dominant.Hbs.of.these.species.has.been.purified.using.Ion-exchange.chromatography.on.CM-cellulose.
column.. Fluorescence. spectrophotometery. studies. showed.
that.the.Hbs.of.these.fishes.have.similar.structural.properties.with.
each. other. and. clear. differences. with. human. Hb.. Comparing. the.
heat. capacity. versus. temperature. of. different. Hbs. profiles. using.
differential. scanning. calorimetry. (DSC). showed. varying. thermal.
unfolding.characteristics..The.percentage.of.alpha-helix.and.betasheet.substructures.has.been.obtained.based.on.circular.dichroism.
analysis.(CD).investigation..UV–vis.spectrophotometer.was.used.
to.measure.oxygen.affinity.using.sodium.dithionite..Oxygen.affinity.and.structural.properties.of.these.Hbs.were.compared.using.Hb.
oxygen.dissociation.curves..
298 Loss of Bacterial MTN Activity Causes Polyamine Deficiency,
KEN CORNELL (Department. of. Chemistry. and. Biochemistry,.
Boise. State. University,. Boise,. ID. 83725-1520;. kencornell@boisestate.edu).
5’. Methylthioadenosine/S-adenosylhomocysteine. nucleosidase.(MTN).is.a.microbe.specific.enzyme.important.in.purine.and.
methionine. salvage. from. byproducts. of. S-adenosylmethionine.
dependent.reactions.such.as.polyamine.synthesis,.biological.methylation,.
autoinducer. and. vitamin. synthesis.. Interruption. of. MTN.
activity.in.E. coli.leads.to.marked.decreases.in.intracellular.polyamine.content.and.decreased.growth.rates.that.are.largely.reversible.
by.polyamine.supplementation..The.altered.polyamine.synthesis.is.
most.likely.the.result.of.product.inhibition.of.polyamine.synthase.
reactions. caused. by. the. accumulation. of. 5’. Methylthioadenosine.
(MTA).. Proteomic. analysis. also. reveals. that. decreased. levels. of.
polyamine.synthases.accompany.MTN.gene.knock-out..Additional.
effects.on.a.wide-range.of.metabolic.pathways.are.found.with.MTN.
deficiency,.underscoring.a.complex.set.of.mechanisms.of.action.that.
will.be.exerted.by.anti-MTN.specific.drugs,.and.suggesting.routes.
of.drug.potentiation.that.could.lead.to.potent.combination.therapies..
299 Exposure to TCDD (Dioxin) Increases Hepatic Stellate Cell
Activation, WENDy A. HARVEy*, COLEy J. DOOLITTLE,
JALISA J. ROBINSON, and KRISTEN A. MITCHELL.
(Department.of.Biological.Sciences,.Boise.State.University,.Boise,.
130
ID.83278-1515;.wendyharvey@u.boisestate.edu).
2,3,7,8-Tetrachlorodibenzo-p-dioxin. (TCDD). is. an. environmental.
pollutant. in. the. family. of. halogenated. aromatic. hydrocarbons..
Exposure. to. TCDD. elicits. a. spectrum. of. toxic. effects,.
many.of.which.are.rooted.in.aberrant.cell.proliferation,.activation.
and. differentiation.. We. have. previously. shown. that. exposure. to.
TCDD. suppresses. hepatocyte. proliferation. in. a. mouse. model. of.
liver. regeneration. induced. by. 70%. partial. hepatectomy. (PH).. In.
the.present. study,.we.tested.the.hypothesis.that.TCDD.alters.the.
activation.of. hepatic.stellate.cells.(HSCs),. which.regulate.regeneration.
through. the. production. of. soluble. mediators.. Mice. were.
treated.with.TCDD.(20.µg/kg).or.peanut.oil.24.hr.prior.to.PH,.and.
plasma.and.liver.tissue.were.collected.after.surgery..Exposure.to.
TCDD.increased.expression.of.the.HSC.activation.marker.alphasmooth.
muscle. actin. and. increased. levels. of. monocyte. chemoattractant.
protein. (MCP)-1,. a. chemokine. abundantly. produced. by.
HSCs.. Likewise,. in. vitro. studies. using. a. human. hepatic. stellate.
cell.line.revealed.that.TCDD.treatment.increased.proliferation.and.
MCP-1.production.and.impaired.the.ability.of.these.cells.to.store.
retinoids�..Hence,.inappropriate.HSC.activation.may.contribute.to.
impaired.liver.regeneration.in.TCDD-treated.mice.through.excessive.production.of.soluble.mediators,.leading.to.enhanced.inflammation.or.suppressed.hepatocyte.proliferation.
300 Expression of Multipotency Markers in Adult Adipocyte-
Derived Stem Cells as a Function of Time,.LAURINE J. SHAH-
MIRIAN 1 * and KRISTINA I. BOSTROM 2 ( 1 Chaminade.College.
Preparatory. High. School,. 7500. Chaminade.Ave,. West. Hills,. CA.
91304;. 2 David.Geffen.School.of.Medicine,.University.of.California,.Los.Angeles
10945.Le.Conte.Avenue,.Suite.3132.Los.Angeles,.
CA.90095;.laurine.shahmirian@yahoo.com).
White. mature. adipocytes. have. been. shown. to. lose. their. fat.
in.culture.and.de-differentiate.into.de-differentiated.(DFAT).cells..
Immediately.after.the.loss.of.lipids,.DFAT.cells.express.several.stem.
cell.markers.as.a.sign.of.multipotency..However,.it.is.not.known.for.
how.many.cell.passages.the.cells.express.stem.cell.markers,.information.that.is.essential.in.defining.how.long.the.cells.can.be.used.
a. source.of.multipotent.cells..To.test.the.longevity.of.DFAT.cell.
expression.of.stem.markers,.mouse.DFAT.cells.were.cultured.for.up.
to.10.passages..Cellular.RNA.was.extracted.after.each.passage,.and.
cells.were.fixed.and.prepared.for.immunostaining.or.fluorescenceactivated.cell.sorting.(FACS)..Expression.was.determined.by.realtime.PCR..
Real-time.PCR.showed.that.expression.of.Oct3/4,.Nanog.and.
SOX2,.pluripotency.markers,.gradually.decreased.to.near.undetectable.levels.over.10.passages..After.~.2.passages,.25%.of.baseline.(passage.0).for.at.least.2.additional.passages..Optimally,.DFAT.cells.
should.be.used.for.regenerative.purposes.no.later.than.two.passages.
after.the.isolation.of.adipocytes.
301 Oncostatin M: Potential Target for Inhibiting Breast Cancer
Metastasis to Bone, CELESTE BOLIN, KEN TAWARA, and