Impact des métaux lourds sur les interactions plante/ver de terre ...
Impact des métaux lourds sur les interactions plante/ver de terre ...
Impact des métaux lourds sur les interactions plante/ver de terre ...
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tel-00486649, <strong>ver</strong>sion 1 - 26 May 2010PARTIE B : MATERIEL ET METHODES...……………………………………………. 29I. PRESENTATION DES COMPOSANTS BIOLOGIQUES……………………....... 29I.1 La <strong>plante</strong> « hyperaccumulatrice » Lantana camara L……………………………. 29I.1.1 Description et caractéristiques…………………………………………… 29I.1.2 Lantana camara et phytoremédiation……………………………………. 30I.2 Le <strong>ver</strong> <strong>de</strong> <strong>terre</strong>, Pontoscolex corethrurus………………………………………… 30I.2.1 Description et caractéristiques……………………………………………. 30I.2.2 Avantages <strong>de</strong> ce <strong>ver</strong>……………………………………………………… 30II. MISE EN PLACE DES EXPERIMENTATIONS………………………………….. 31II.1 Site d’étu<strong>de</strong>……………………………………………………………………… 31II.2 Matériel.…………………………………………………………………………. 32II.2.1 Les pots.………………………………………………………………… 32II.2.2 Le sol……………………………………………………………………. 32II.2.3 La <strong>plante</strong>………………………………………………………………… 33II.2.4 Le <strong>ver</strong> <strong>de</strong> <strong>terre</strong>…………………………………………………………… 33II.2.5 Le plomb………………………………………………………………… 34II.3 Plan <strong>de</strong> l’expérience…………………………………………………………....... 34III. DEMONTAGE DES MICROCOSMES……………………………………………... 36III.1 La <strong>plante</strong>………………………………………………………………………... 36III.2 Les échantillons <strong>de</strong> sol…………………………………………………………. 36IV. ANALYSES PHYSIQUES ET CHIMIQUES………………………………………. 38IV.1 Propriétés physiques et chimiques…………………………………………….... 38IV.2 Teneur en plomb <strong><strong>de</strong>s</strong> <strong>plante</strong>s……………………………………………………. 38V. TECHNIQUES MICROBIOLOGIQUES………………………………………….... 39V.1 Dosages enzymatiques…………………………………………………………... 39V.1.1 Préparation <strong><strong>de</strong>s</strong> solutions <strong>de</strong> sol………………………………………… 39V.1.2 Dosage <strong><strong>de</strong>s</strong> phosphatases aci<strong>de</strong> et alcaline…………………………….... 39V.1.3 Dosage <strong><strong>de</strong>s</strong> polysacchari<strong><strong>de</strong>s</strong>…………………………………………….. 39V.1.4 Dosage <strong><strong>de</strong>s</strong> hétérosidases……………………………………………….. 40V.1.5 Dosage <strong><strong>de</strong>s</strong> uréases…………………………………………………….... 40V.1.6 Dosage <strong>de</strong> la FDA………………………………………………………. 40
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