8th INTERNATIONAL WHEAT CONFERENCE

were clear differentiated. For RAPD-PCR analyses have been used 17 effective primers
and assessed the intra-specie and inter-specie genetic diversity of the Triticum aestivum.
According phenotypical traits as grain color, ear color, and awn, ear and steam, fragility,
the intra-species diversity have been observed. The main fragment separation zone where
within 2000-200 b.p. In whole, the 273 amplified fragments from 12 to 23 (16, in average)
to one RAPD primer. Among 273 PCR fragments 54% were polymorphic between
investigated genotypes, 46% have the similar length among all investigated samples. All
primers in the different correlations established the monomorphic and polymorphic fragments
between investigated samples. The monomorphic fragments can be considered
as RAPD-markers for specie representatives. The genetic distances between investigated
samples the more the less common amplified products. Revealed monomorphic bands of
different varieties speculate similarity of the structural-functional organization of their
genome. Each variety have own specific spectrum of the RAPD products, differed from
each other by fragment quantity, their size and manifestation rate. Some primers revealed
specific to their variety amplicons, however, it would be specific varietal characteristics.
Significant differences on RAPD fragments quantities have been marked. The investigated
samples have been differed in the numbers of the unique amplicons characterized
for each variety. Based on the cluster analyses the dendrogram of the genetic similarity
between investigated wheat samples has been constructed. The dendrogram have been
revealed the high level of the intra-specific polymorphism.
Therefore, molecular-genetic analyses give the possibility to reveal the specific genome
markers, which can be used for varietal genome identifications. It shown, that the method
of molecular marking based on RAPD-PCR allowed to identify the representatives of the
Triticum aestivum species and to create phylogenetic links between different varieties.
Unequivocal use of the varietal specific primers allowed to reducing expenditure and
inputs which are necessary for collection analyses.
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