8th INTERNATIONAL WHEAT CONFERENCE

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BReedINg foR eNhANCed gRAIN-zINC ANd IRoN CoNCeNTRATIoNS IN CImmyT SPRINg BReAd WheAT geRmPLASm G. Velu 1 , R.P. Singh 1 , J. Huerta-Espino 2 , R.J. Peña 1 , I. Ortiz-Monasterio 1 , S. Bhavani 1 , S.A. Herrera-Foessel 1 and P.K. Singh 1 1 International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico DF, Mexico; 2 Campo Experimental Valle de Mexico INIFAP, Apdo. Postal 10, 56230 Chapingo, Edo de Mexico, Mexico E-mail: velu@cgiar.org Micronutrient malnutrition arising from zinc (Zn) and iron (Fe) deficiency has emerged as a serious health concern worldwide. Genetic enhancement of crops with elevated levels of these micronutrients is one of the most cost effective ways of solving global micronutrient malnutrition problem. The HarvestPlus Challenge Program (http://www.harvestplus. org) of the Consultative Group on International Agricultural Research (CGIAR) supports breeding of bio-fortified cultivars with enhanced levels of micronutrients in major staples, including wheat (Triticum aestivum L.). Development and dissemination of high Zn and Fe containing high-yielding, disease-resistant wheat varieties by CIMMYT and South Asian NARS partners is initially targeted for the Indo-Gangetic plains, a region with high population density and high micronutrient malnutrition. The most promising sources for grain Zn and Fe concentrations are wild relatives, primitive wheats and landraces. Synthetic hexaploids were developed at CIMMYT by crossing Aegilops taushii and high Zn and Fe containing accessions of T. dicoccom. Current breeding efforts at CIMMYT have focused in transferring genes governing increased Zn and Fe from T. spelta and T. dicoccom based synthetics, land races and other reported high Zn and Fe sources to high yielding elite wheat backgrounds. Limited backcross (BC1 and BC2) populations of between 400 to 800 plants with elite materials and subsequent F2 (1200–2400 plants) and F3-F4 (400–800 plants) are grown and plants with desired agronomic features selected. A total of 3732 BC1 derived F4 and BC2 derived F3 lines were grown in Cd. Obregon, Mexico during 2008-09 with checks in soil enriched with Zn fertilizer. We retained 1300 F5 and F4 lines after selection for agronomic characteristics and resistance to leaf and stem rust. These lines were analyzed for grain Zn/Fe concentrations, and evaluated for agronomic characteristics and resistance to leaf and yellow rust in El Batan and Toluca, Mexico, respectively during 2009 summer season. More than 600 promising F6/F5 lines, with +1 standard deviation higher Zn/Fe concentrations over the mean and desirable agronomic characteristics and disease resistance, are under yield tests in replicated trials in Cd. Obregon, Mexico during 2009-2010 crop season in Zn enriched soil. In addition, 5–10 heads were harvested from each of the selected line and 4795 F6/ 554
F5 head-rows of were also planted in Zn enriched soil to obtain homogenous advanced generation derivatives. Based on the yield performance, disease resistance and high grain Zn/Fe concentrations, head-rows of the outstanding lines will be promoted for advanced yield trials and further testing in target countries. A similar strategy, however restricted to BC1, is being followed in developing new breeding populations that involve additional high Zn and Fe sources. 555
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Borlaug Global Rust Initiative
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© 2010, N.I. Vavilov Research Inst
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Koppel Reine, Ingver Anne Requireme
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A.V. Konarev, T.I.Peneva, N.K.Gubar
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Majed M.A Al-Bokari, Saad M. Alzahr
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Ratan Tiwari, Rajender Singh, Sindh
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Kadir Akan, Zafer Mert, Lütfi Çet
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Lapochkina I. F., Gajnullin N. R.,
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Victoria A. Valdez, Scott D. Haley,
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Ribas Vargas, G, Reynolds, M, De Si
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RM DePauw, RE Knox, JB Thomas, DG H
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Paramjit Khurana and Harsh Chauhan
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Vázquez, D.; Berger, A.; Cuniberti
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Sedláček Tibor Prediction of baki
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that some crops, such as wheat, oat
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ferent continents, Asia and Europe.
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A STRATegIC Look AT gLoBAL WheAT PR
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it would be logical to conclude tha
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• Rational retention of adequate
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STudy of RAIN effeCTS oN RAINfed WI
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amount of durum and carotenoid rich
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evaluation under farmers’ conditi
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The fACToRS effeCTINg The RegIoNAL
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AdoPTIoN of CoNSeRVATIoN AgRICuLTuR
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Severe disease attacks are not the
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Coefficients of variation (CV) of t
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humidity (67 and 80%) and occurrenc
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need to be qualitatively evaluated
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Strengthening the capacity of wheat
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CoNSeRVATIoN AgRICuLTuRe foR SuSTAI
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BReedINg WheAT foR ReduCed ImPACT o
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WheAT ImPRoVemeNT ChALLeNgeS ANd oP
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PLeNARy SeSSIoN 2: uTILIzATIoN of W
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uTILIzATIoN of NeW WheAT geNePooL I
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IdeNTIfICATIoN of SouRCeS of SeedLI
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CoNSeRVINg CRoP dIVeRSITy IN The 21
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Genetic analysis of leaf rust resis
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T.aestivum-H.villosa interstitial t
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Growing F2-F4 generations as bulks.
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ferences among accessions. Differen
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dIgITAL PhoTogRAPhy AS A NoN-deSTRu
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BReAd WheAT (TRITICum AeSTIVum, L)
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eVALuATIoN ANd uTILIzATIoN of WheAT
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TRANSLoCATIoNS IN INTRASPeCIfIC kAR
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WheAT LANdRACeS ANd oBSoLeTe CuLTIV
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TRANSfeR of WheAT STReAk moSAIC VIR
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BReedINg fRee-ThReShABLe, dWARf emm
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geNeTIC STRuCTuRe of AegILoPS TAuSC
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PLANT geNeTIC ReSouRCeS IN CeNTRAL
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uSe of TRITICeAe TRIBe SPeCIeS foR
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moLeCuLAR mARkeRS foR INCReASINg ef
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ChARACTeRISTICS of WheAT geNeTIC Re
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moLeCuLAR-CyTogeNeTIC ChARACTeRIzAT
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moLeCuLAR CyTogeNeTIC ChARACTeRIzAT
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gRAIN yIeLd PeRfoRmANCe of SyNTheTI
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ANALySIS of The PASSPoRT INfoRmATIo
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INdIReCT SeLeCTIoN uSINg RefeReNCe
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geNome ANALySeS of The ABoRIgINe LA
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SImuLTANeouS ReSISTANCe To PoWdeRy
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PheNoTyPIC dIVeRSITy IN SPANISh LAN
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highly resistant to English grain a
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SPANISh LANdRACeS of TRITICum TuRgI
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No chromosome rearrangements were d
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uTILIzATIoN of eLyTRIgIA INTeRmedIA
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WheAT ImPRoVemeNT uSINg Rye - dISTu
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WheAT-PSAThyRoSTAChyS huAShANICA Ch
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Stagonospora nodorum blotch (Stagon
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cific resistance to pyrenophorosis,
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uNdeRSTANdINg gRAIN yIeLd: IT IS A
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gRoWTh, yIeLd ANd PhoToSyNTheTIC Re
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eNhANCemeNT of heAT ToLeRANCe IN Wh
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SALINITy ToLeRANCe ANd NA + exCLuSI
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IdeNTIfICATIoN ANd dISTRIBuTIoN of
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SeLeCTIoN of TeRmINAL dRoughT ToLeR
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yIeLd STABILITy ANd PeRfoRmANCe of
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Like Lona at normal soil water leve
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versus sister lines carrying Rht-B1
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eVALuATIoN of quANTITATIVe ChARACTe
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RoLe of WheAT geNome IN ALumINIum T
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ASSoCIATIoN of RooT WATeR-uPTAke WI
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filling period. Halna showed very l
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effeCT of defICIT IRRIgATIoN IN dIf
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Al-tolerant genotypes maintained lo
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to imperatives of climate change, e
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CoLLeCTIoN of ePI-LINeS of CommoN W
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the field under ideal and late sowi
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A SImPLe ANd effICIeNT TWo STeP mAN
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Furthermore, F V /F M is particular
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ALLeLIC dIVeRSITy foR VeRNALIzATIoN
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PoSSIBILITy of uSINg RooT gRoWTh AN
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ACCumuLATIoN of PhoToSyNTheSIS ASSI
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(1 scores - low hardiness; 9 scores
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exPReSSIoN of Seed doRmANCy IN CRoA
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eVALuATIoN of WheAT SyNTheTIC hexAP
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CoLeoPTILe LeNgTh of Some WheAT VAR
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nitrogen supply and soil water avai
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IdeNTIfICATIoN of SouRCeS foR heAT,
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PhySIoLogIC ANd BIoChemICAL ReSPoNS
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ReSuLTS of eVALuATIoN of SPRINg Whe
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BReedINg foR ImPRoVed ToLeRANCe IN
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STRATegIeS foR ImPRoVINg WheAT ReSI
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deVeLoPmeNT of ISogeNIC LINeS foR R
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BReedINg STRATegIeS foR fuSARIum he
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INCReASed ToLeRANCe To WheAT PoWdeR
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The homoeoLogouS RegIoNS oN LoNg AR
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WAyS To ImPRoVe Weed ComPeTITIVe AB
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fhB ReSISTANT modeL VARIeTy of NoRT
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mININg A CoLLeCTIoN of WheAT LANdRA
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IdeNTIfICATIoN of SouRCeS of ReSIST
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ChARACTeRIzATIoN of STem RuST ReSIS
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The 2 nd generation slow-rusters ha
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virus complex present across the fi
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During the same years grain quality
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mAPPINg of AduLT PLANT STem RuST Re
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IdeNTIfICATIoN of geNeTIC ReSISTANC
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APPLyINg mALe STeRILITy medIATed mA
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etween these parameters, indicating
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the field and one in pots placed ou
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ReSeARCh oN INheRITANCe of yeLLoW R
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ReSISTANCe BReedINg AgAINST LeAf SP
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PoWdeRy mILdeW ANd LeAf RuST ReSIST
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oLd CzeCh WheAT CuLTIVARS AS A NeW
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isk will vary depending on the path
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to a number of countries including
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geNeTIC dIVeRSITy of The fuNgAL Whe
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accessions, producing durum wheat w
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ReLATIoNShIPS BeTWeeN fuSARIum heAd
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PoPuLATIoN STRuCTuRe of PuCCINIA TR
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LeAf RuST ReSISTANCe geNeS LR10, LR
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ComBININg WheAT RuST ANd fuSARIum h
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LeAf RuST ReSISTANCe geNe LR67, A T
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STATuS of WheAT PAThoSySTemS fRom 1
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WkS1 (WheAT kINASe START) LImITS gR
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quANTITATIVe TRAIT LoCI foR ReSISTA
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geNeTIC ANALySIS of STem RuST ReSIS
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geNeTIC ImPRoVemeNT of WheAT ReSIST
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ChARACTeRIzATIoN of A NoVeL gLuTeNI
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effeCT of WheAT CuLTIVARS oN VARIAB
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dIVeRSITy of geNeS of ReSISTANCe To
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TAke-ALL ReSISTANCe of euRoPeAN WIN
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dISCoVeRy of TWo quANTITATIVe ReSIS
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Parula had leaf rust severity betwe
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quANTITATIVe PCR AS A TooL foR TILL
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quANTITATIVe TRAIT LoCI ASSoCIATed
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gLoBAL INITIATIVeS foR mANAgemeNT o
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deoxyNIVALeNoL LeVeL IN WheAT gRAIN
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VIRuLeNCe STRuCTuRe of The WheAT ST
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ReLeASe STem RuST ReSISTANT VARIeTI
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AN ASSoCIATIoN geNeTICS APPRoACh To
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cantly greater number of lines (59)
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The INTRoduCTIoN INTo WINTeR WheAT
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effeCTIVe yeLLoW RuST ReSISTANCe ge
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geNeTICS of ReSISTANCe To RACe TTkS
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partially resistant as these genoty
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The moST ImPoRTANT WheAT dISeASeS I
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SPRINg WheAT BReedINg foR LeAf ANd
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CommoN RooT RoT ReACTIoN IN A dIVeR
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hAPLoTyPe ANd ASSoCIATIoN ANALySeS
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IdeNTIfICATIoN of A NeW ReSISTANCe
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mARkeR-ASSISTed SeLeCTIoN foR LeAf
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ReACTIoN of ToLeRANT WheAT geNoTyPe
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that changes in the composition of
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deVeLoPmeNT of PCR-BASed mARkeRS fo
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BIoCoNTRoL of fuSARIum heAd BLIghT
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ImPRoVINg WheAT STRIPe RuST ReSISTA
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BReedINg AduLT PLANT ReSISTANCe To
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improve localisation of QTL positio
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ContRibutions of CRoP Physiology to
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ToWARdS The uNdeRSTANdINg of geNeTI
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SPIke gRoWTh ReguLATINg RATe of deA
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quANTIfyINg hoW WINTeR WheAT CRoPS
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domeSTIC WheAT PRoduCTIoN ANd fuTuR
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for genetic enhancement through int
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IdeNTIfICATIoN of duRum WheAT geNoT
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GPC varied significantly by effect
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geNeTIC VARIABILITy of NITRogeN uSe
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effICIeNCy ANd ChALLeNgeS of PReCIS
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AChIeVemeNTS of WheAT BReedINg AT d
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vs. 7.8 in S1). Grain number explai
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ImPACT of CImmyT PRogRAmS oN WheAT
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gRAIN yIeLd ANd quALITy of WINTeR W
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BReedINg of WheAT IN kyRgyzSTAN ANd
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WheAT fLoReT deVeLoPmeNT IN ReSPoNS
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IdeNTIfyINg NoVeL TRAITS ANd geNeTI
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IdeNTIfICATIoN of TRAITS To ImPRoVe
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geNoTyPe ANd TeSTINg SITe eVALuATIo
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dWARfINg (RhT) ANd PhoToPeRIod INSe
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SeLeCTINg WINTeR WheAT geNoTyPeS fo
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APPLICATIoN of geNoTyPe x eNVIRoNme
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ReSeARCh AdVANCe of The ChoNgqINg-T
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INTeRNAL moRPhoLogICAL ChANgeS of f
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geNeTIC PRogReSS IN WheAT yIeLd ANd
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WheAT BReedINg IN TAJIkISTAN Muminj
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yIeLd ANd Some PhySIoLogICAL ChARAC
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CRoP yIeLd IN duRum WheAT ANd The P
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SySTem APPRoACh To ANALySIS of yIeL
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ChANgeS of SPIke ARChITeCTuRe duRIN
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to that of Sr36 cultivars. The powd
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seed is produced by breeders of the
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effeCTS of SPeCIfIC RhT ANd PPd ALL
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mAIN dIReCTIoNS of AdAPTIVe BReedIN
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PhoToPeRIod SeNSITIVITy IN duRum Wh
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elongatum through cultivar Teewon,
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increase yield above 30% or 9.755t/
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mostly was used to synthesize prote
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geNomIC TeChNoLogIeS ANd ReSouRCeS
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NeW BReedINg TooLS ImPACT CANAdIAN
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geNoTyPIC STRuCTuReS of The CImmyT
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INSeRTIoN SITe-BASed PoLymoRPhISm (
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has been established at the Nationa
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geNomIC ABuNdANCe of VARIouS SImPLe
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STRuCTuRAL ReARRANgemeNTS of WheAT
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geNeRAL STATISTICAL PoWeR of NeSTed
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LoCALIzATIoN of qTLS foR AgRoNomIC
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uSINg mARkeR ASSISTed SeLeCTIoN IN
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PoPuLATIoN STRuCTuRe IN A CoRe CoLL
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was observed. Cultivars that belong
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A QTL was considered to be “stabl
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deVeLoPINg A ReCuRReNT SeLeCTIoN PR
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hIgh effICIeNCy TRANSfoRmATIoN of h
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deTeCTIoN of dNA PoLymoRPhISmS ASSo
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IdeNTIfICATIoN ANd ANALySIS of TAWR
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geNeTIC modIfICATIoN of PhoToSyNThe
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isolated were mapped to the chromos
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stress. Transgenic Arabidopsis plan
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geRmPLASm eNhANCemeNT ANd NeW VARIe
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(174%) than in pedigree schemes (21
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moLeCuLAR CyTogeNeTIC ChARACTeRIzAT
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PhySIoLogICAL ANd BIoChemICAL APPRo
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ChARACTeRIzATIoN of The geNeTIC VAR
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ToWARdS A PhySICAL mAP of SST1, A m
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effICIeNT INCoRPoRATIoN of AduLT PL
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deVeLoPmeNT ANd APPLICATIoN of TRIT
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uSe of hIgh moLeCuLAR WeIghT gLuTeN
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foRmATIoN of geNeTIC VARIABILITy AT
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group were rough with difficult thr
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VALIdATIoN of 86 moLeCuLAR mARkeRS
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genetiC vARiAtion of gli-2 in kAzAk
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of DNA sequence variations sometime
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VARIABILITy of SALINITy ToLeRANCe R
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484
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ImPRoVINg The heALTh BeNefITS of Wh
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hARVeSTPLuS: A gLoBAL effoRT To INC
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deVeLoPmeNT of WheAT geNoTyPeS WITh
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dyNAmIC ChANge of gRAIN SugAR, STAR
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component analysis performed on qua
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associated to the cultivar effect.
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and diversity associated with quali
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levels. It suggests that it is easi
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geNeTIC VARIABILITy of duRum WheAT
Page 506 and 507: uTILIzATIoN of oLd WheAT VARIeTIeS
Page 508 and 509: The effeCTS of gPC-B1 oN gRAIN PRoT
Page 510 and 511: ISoLATIoN of dIffeReNTIALLy exPReSS
Page 512 and 513: eVALuATIoN meThod foR quALITy ChARA
Page 514 and 515: in 14 out of 41 specifications (34.
Page 516 and 517: and no significant effect of Glu-A3
Page 518 and 519: PSy-A1 ALLeLeS ANd oTheR geNeS CoNT
Page 520 and 521: mAPPINg qTLS foR LIPoxygeNASe ACTIV
Page 522 and 523: The mean value of LV at low N rate
Page 524 and 525: CuRReNT STATuS ANd PeRSPeCTIVeS foR
Page 526 and 527: INTeRACTIoN effeCTS of eNVIRoNmeNTA
Page 528 and 529: this study). In terms of gluten str
Page 530 and 531: Second problem that had to be solve
Page 532 and 533: dough VISCo-eLASTIC PRoPeRTIeS of R
Page 534 and 535: gRAIN quALITy of WINTeR WheAT CuLTI
Page 536 and 537: BReedINg WheAT foR hIgh ToCoL CoNTe
Page 538 and 539: qTL ANALySIS of PRINCIPAL ANd ePIST
Page 540 and 541: PRedICTIoN of BAkINg quALITy By SoL
Page 542 and 543: Ukraine has two geographical-climat
Page 544 and 545: acid between 16 and 21 mg/g, vanill
Page 546 and 547: Raweta cv. by 10%. Grains of invest
Page 548 and 549: for both traits. Besides, group of
Page 550 and 551: Five pairs of epistatic effects wer
Page 552 and 553: odies, which could form either by d
Page 554 and 555: References Lewis S., Bullrich L., K
Page 558 and 559: geNomIC ANd PRoTeomIC STudIeS of gL
Page 560 and 561: 558
Page 562 and 563: © 2010, N.I. Vavilov Research Inst
Page 564 and 565: INTERRELATIONSHIPS OF AGRONOMIC CHA
Page 566 and 567: MULTIVARIATE ANALYSIS OF GENETIC DI
Page 568 and 569: A METHOD OF ASSESSING SPIKE FERTILI
Page 570: IDENTIFICATION OF LMW GLUTENIN SUBU
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8th INTERNATIONAL WHEAT CONFERENCE

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