8th INTERNATIONAL WHEAT CONFERENCE

RoLe of WheAT geNome IN ALumINIum ToLeRANCe
of TRITICALe
Agnieszka Fiuk, Piotr Tomasz Bednarek, Andrzej Anioł
Plant Breeding and Acclimatization Institute, Department
of Plant Physiology and Biochemistry,
Radzików, 05-870 Błonie, Poland
E-mail Address of presenting author: afiuk@ihar.edu.pl
Crop cultivation, due to increasing acreages of acidic soils (over 40% of agricultural area) faces
the problem of aluminium toxicity leading to the reduction of seed yield. Thus, evaluation of
aluminium (Al) tolerant forms is an urgent aspect of modern breeding. Although putative
chromosomal location of Al tolerant genes was identified in case of barley, rye and wheat,
their function is mostly unknown while some of them belong to ALMT (aluminium-activated
malate transporter) and MATE (multidrug and toxin efflux) families and are known to be responsible
for malate and citrate secretion, respectively. Molecular markers linked to the genes
and described in the literature are hardly useful for marker assisted selection (MAS) since they
are either apart from the genes or were developed on a specific genetic background.
Triticale (x Triticosecale Wittmack) is a synthetic wheat/rye hybrid that exhibits adaptation to
adverse environmental conditions and may more efficiently utilize nutrients as compared to
wheat or barley. However its yield is affected by acidic soils. In triticale chromosomal location
of Al tolerant genes is not known. Nevertheless it seems that in octoploids the wheat genome
plays the clue role (Stass et al., 2008), while in hexaploid forms it may originate from rye (Ma
et al. 2000). The molecular markers linked to the genes of interest are still not known. Thus, the
aim of the study was the identification of markers linked to Al-tolerant genes based on mapping
F2 populations of hexaploid triticale, assignment of the linkage groups to the chromosomes and
verification whether wheat or rye genome is the donor one for the tolerant gene.
Two F2 mapping populations (P1 and P15) derived from the crosses of tolerant double
haploid plants cv. Bogo with opposite Al-tolerance were used in molecular experiments.
Plant materials (seed of F2 populations) were planted under different environmental conditions
(field and greenhouse) following fingerprinting of individuals. Twenty AFLP selective
primer combinations, 215 rye and 45 wheat microsatellite primers and DArT markers were
used. In case of P1 and P15 populations eight and nine groups were identified, two linkages
were located on rye chromosomes 5R and 7R, and one on wheat chromosome 2B. Interval
mapping assigned Al-tolerance to the chromosome 7R and suggested that two markers
were about 2 cM apart from the maximum of LOD function. The same markers were also
indicated by association mapping as the markers associated with the trait.
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