8th INTERNATIONAL WHEAT CONFERENCE

deVeLoPmeNT ANd APPLICATIoN of TRITICum
TImoPheeVII mAPPINg dATA IN WheAT ReSISTANCe
mANAgemeNT STRATegIeS
E.A. Salina 1 , I.N. Leonova, E.M. Egorova, E.B. Budashkina,
M. Röder 2
1 Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
2 Leibniz Institute of Plant Genetics and Crop Research, Gatersleben, Germany
E-mail Address of presenting author: salina@bionet.nsc.ru
One of the approaches of modern plant biotechnology is application of the genes and
genomes mapping data for direct introgression of agronomic valuable traits into recipient
genotypes. Wild relatives of common wheat and Triticum timopheevii are regularly
involved in breeding process as donors for creation of new forms of wheat with the improved
properties.
The following researches were conducted for more effective using of the donor species
T. timopheevii in biotechnological wheat programs:
Construction of molecular-genetic maps of T. timopheevii by using microsatellite
(SSR) markers.
Genetic maps for the two crosses T. timopheevii var. timopheevii × T. timopheevii var.
typica and T. timopheevii K-38555 × T. militinae were constructed. On the first population,
121 SSR loci were mapped, and on the second population 103 SSR loci.
Identification of the chromosomal regions determining disease resistances in T. aestivum
х T. timopheevii introgression lines through SSR-genotyping.
Genetic analysis indicated that leaf rust resistance of wheat introgression lines (T.
aestivum x T. timopheevii) lines is defined by more than one gene. Molecular analysis
with genome-specific microsatellite markers revealed localization of the T. timopheevii
genome fragments in introgression line 832 in chromosomes 1A, 2A, 2B, 5A and 6B.
Mapping of disease resistance genes.
Molecular-genetic mapping demonstrated that resistance of the line-832 is determined
by three loci on chromosomes 1А (QLr.icg-1A), 2А (QLr.icg-2A) and 5В (QLr.
icg-5B) responsible for 10, 11 and 54% of phenotypic variance, respectively.
Application of marker assisted selection for development resistant near-isogenic lines
of common wheat.
To create a series of lines carrying the major loci QLr.icg-5B, line 832 was backcrossed three
times to common wheat cultivar Saratovskaya 29. Selection of plants containing QLr.icg-
5B loci was performed in BC 2 F 1 and BC 3 F 1 progenies with microsatellites specific for T.
aestivum (BBAADD) and T. timopheevii (GGA t A t ) genomes. The near isogenic lines were
additionally analyzed by means of in situ hybridization with probes Spelt1 and pSc119.2 located
in B and G genomes. As a result, six lines carrying QLr.icg-5B were developed and leaf
rust resistance of the lines was determined at seedling and adult plant stages. It was shown
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