8th INTERNATIONAL WHEAT CONFERENCE

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TRANSfeRRINg uSefuL Rye geNeS To WheAT, uSINg TRITICALe AS A BRIdge N.N. Saulescu, G. Ittu, M. Ciuca, M. Ittu, P. Mustatea National Agricultural Research and Development Institute Fundulea, 915200 ROMANIA E-mail Address of presenting author: saulescu@incda-fundulea.ro Increased genetic diversity in wheat breeding is desirable for dealing with present and future challenges caused by the need for increased yields, by climate changes and by higher consumer concerns for food safety. Rye has already proved to be a good donor of genes for improving important traits and diversity in wheat breeding. Over 300 wheat cultivars carrying wheat-rye translocations, particularly involving the short arm of rye chromosome 1R (1RS), have been released worldwide. Wheat-rye translocations determine a number of useful characteristics such as disease resistance (powdery mildew, stem rust, leaf rust and stripe rust) or tolerance to barley yellow dwarf virus and insect resistance (Hessian fly, Russian wheat aphid, green bug) and have been reported to improve yield potential, stress tolerance, and adaptation in bread wheat. Wheat–rye translocations that proved potential in breeding for resistance or tolerance to biotic or abiotic stresses are first of all 1BL.1RS and 1AL.1RS, but also 2BS.2 RL, 5AS.5RL, and 6BS.6RL. The agronomic advantages of these translocations, as well as their pleiotropic detrimental effects, mainly on quality, proved to be dependent on the wheat genetic background, wheat class and environmental condition, but also on the source of the transferred rye chromatin. This justifies continued effort for introgression of rye genes from various sources into various wheat backgrounds. Besides, there are still many other genes of interest for wheat improvement, not yet transferred, that are available in the rye genome. To take advantage of the existence at National Agricultural Research & Development Institute Fundulea, Romania of intensive breeding programs in both winter wheat and triticale, a strategy for introgression of useful rye genes to wheat, using triticale as a bridge, has been applied in our program the since 1999. Using Triticale as a bridge has several advantages such as: taking advantage of the large genetic progress made in triticale breeding, relatively easy crossing of triticale with wheat and selection of potentially useful rye genes based on their expression in the presence of two wheat genomes. Our strategy includes: Large scale crossing of best hexaploid triticales with best wheat cultivars Backcross with wheat. Grains obtained by open pollination of F1 plants surrounded by wheat are also harvested and used for planting segregating populations. 78
Growing F2-F4 generations as bulks. Occasionally outstanding wheat like plants are selected beginning from F2. Selection of wheat-like plants and progenies with traits potentially controlled by rye genes. Detecting potential rye genes introgressions using rye specific SSR markers Identification of rye introgressions using chromosome specific markers and FISH Use of identified lines of interest as parents in further crosses and selection of progenies carrying the desired trait using phenotypic selection and specific markers. First results of this approach will be presented, including: A line, carrying a 1AL.1RS translocation, carrying a potentially new gene for resistance to common bunt, Lines with good tolerance to BYDV Lines with good Septoria tritici and Puccinia recondita resistance Lines with improved albedo Lines with improved allelopathy 79
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Borlaug Global Rust Initiative
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© 2010, N.I. Vavilov Research Inst
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Koppel Reine, Ingver Anne Requireme
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A.V. Konarev, T.I.Peneva, N.K.Gubar
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Majed M.A Al-Bokari, Saad M. Alzahr
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Ratan Tiwari, Rajender Singh, Sindh
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Kadir Akan, Zafer Mert, Lütfi Çet
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Lapochkina I. F., Gajnullin N. R.,
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Victoria A. Valdez, Scott D. Haley,
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Ribas Vargas, G, Reynolds, M, De Si
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RM DePauw, RE Knox, JB Thomas, DG H
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Paramjit Khurana and Harsh Chauhan
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Vázquez, D.; Berger, A.; Cuniberti
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Sedláček Tibor Prediction of baki
Page 31 and 32: that some crops, such as wheat, oat
Page 33: ferent continents, Asia and Europe.
Page 36 and 37: A STRATegIC Look AT gLoBAL WheAT PR
Page 38 and 39: it would be logical to conclude tha
Page 40 and 41: • Rational retention of adequate
Page 42 and 43: STudy of RAIN effeCTS oN RAINfed WI
Page 44 and 45: amount of durum and carotenoid rich
Page 46 and 47: evaluation under farmers’ conditi
Page 48 and 49: The fACToRS effeCTINg The RegIoNAL
Page 50 and 51: AdoPTIoN of CoNSeRVATIoN AgRICuLTuR
Page 52 and 53: Severe disease attacks are not the
Page 54 and 55: Coefficients of variation (CV) of t
Page 56 and 57: humidity (67 and 80%) and occurrenc
Page 58 and 59: need to be qualitatively evaluated
Page 60 and 61: Strengthening the capacity of wheat
Page 62 and 63: CoNSeRVATIoN AgRICuLTuRe foR SuSTAI
Page 64 and 65: BReedINg WheAT foR ReduCed ImPACT o
Page 66 and 67: WheAT ImPRoVemeNT ChALLeNgeS ANd oP
Page 69 and 70: PLeNARy SeSSIoN 2: uTILIzATIoN of W
Page 71 and 72: uTILIzATIoN of NeW WheAT geNePooL I
Page 73 and 74: IdeNTIfICATIoN of SouRCeS of SeedLI
Page 75 and 76: CoNSeRVINg CRoP dIVeRSITy IN The 21
Page 77 and 78: Genetic analysis of leaf rust resis
Page 79: T.aestivum-H.villosa interstitial t
Page 83 and 84: ferences among accessions. Differen
Page 85 and 86: dIgITAL PhoTogRAPhy AS A NoN-deSTRu
Page 87 and 88: BReAd WheAT (TRITICum AeSTIVum, L)
Page 89 and 90: eVALuATIoN ANd uTILIzATIoN of WheAT
Page 91 and 92: TRANSLoCATIoNS IN INTRASPeCIfIC kAR
Page 93 and 94: WheAT LANdRACeS ANd oBSoLeTe CuLTIV
Page 95 and 96: TRANSfeR of WheAT STReAk moSAIC VIR
Page 97 and 98: BReedINg fRee-ThReShABLe, dWARf emm
Page 99 and 100: geNeTIC STRuCTuRe of AegILoPS TAuSC
Page 101 and 102: PLANT geNeTIC ReSouRCeS IN CeNTRAL
Page 103 and 104: uSe of TRITICeAe TRIBe SPeCIeS foR
Page 105 and 106: moLeCuLAR mARkeRS foR INCReASINg ef
Page 107 and 108: ChARACTeRISTICS of WheAT geNeTIC Re
Page 109 and 110: moLeCuLAR-CyTogeNeTIC ChARACTeRIzAT
Page 111 and 112: moLeCuLAR CyTogeNeTIC ChARACTeRIzAT
Page 113 and 114: gRAIN yIeLd PeRfoRmANCe of SyNTheTI
Page 115 and 116: ANALySIS of The PASSPoRT INfoRmATIo
Page 117 and 118: INdIReCT SeLeCTIoN uSINg RefeReNCe
Page 119 and 120: geNome ANALySeS of The ABoRIgINe LA
Page 121 and 122: SImuLTANeouS ReSISTANCe To PoWdeRy
Page 123 and 124: PheNoTyPIC dIVeRSITy IN SPANISh LAN
Page 125 and 126: highly resistant to English grain a
Page 127 and 128: SPANISh LANdRACeS of TRITICum TuRgI
Page 129 and 130: No chromosome rearrangements were d
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uTILIzATIoN of eLyTRIgIA INTeRmedIA
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WheAT ImPRoVemeNT uSINg Rye - dISTu
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WheAT-PSAThyRoSTAChyS huAShANICA Ch
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Stagonospora nodorum blotch (Stagon
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cific resistance to pyrenophorosis,
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uNdeRSTANdINg gRAIN yIeLd: IT IS A
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gRoWTh, yIeLd ANd PhoToSyNTheTIC Re
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eNhANCemeNT of heAT ToLeRANCe IN Wh
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SALINITy ToLeRANCe ANd NA + exCLuSI
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IdeNTIfICATIoN ANd dISTRIBuTIoN of
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SeLeCTIoN of TeRmINAL dRoughT ToLeR
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yIeLd STABILITy ANd PeRfoRmANCe of
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Like Lona at normal soil water leve
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versus sister lines carrying Rht-B1
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eVALuATIoN of quANTITATIVe ChARACTe
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RoLe of WheAT geNome IN ALumINIum T
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ASSoCIATIoN of RooT WATeR-uPTAke WI
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filling period. Halna showed very l
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effeCT of defICIT IRRIgATIoN IN dIf
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Al-tolerant genotypes maintained lo
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to imperatives of climate change, e
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CoLLeCTIoN of ePI-LINeS of CommoN W
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the field under ideal and late sowi
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A SImPLe ANd effICIeNT TWo STeP mAN
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Furthermore, F V /F M is particular
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ALLeLIC dIVeRSITy foR VeRNALIzATIoN
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PoSSIBILITy of uSINg RooT gRoWTh AN
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ACCumuLATIoN of PhoToSyNTheSIS ASSI
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(1 scores - low hardiness; 9 scores
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exPReSSIoN of Seed doRmANCy IN CRoA
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eVALuATIoN of WheAT SyNTheTIC hexAP
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CoLeoPTILe LeNgTh of Some WheAT VAR
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nitrogen supply and soil water avai
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IdeNTIfICATIoN of SouRCeS foR heAT,
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PhySIoLogIC ANd BIoChemICAL ReSPoNS
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ReSuLTS of eVALuATIoN of SPRINg Whe
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BReedINg foR ImPRoVed ToLeRANCe IN
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STRATegIeS foR ImPRoVINg WheAT ReSI
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deVeLoPmeNT of ISogeNIC LINeS foR R
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BReedINg STRATegIeS foR fuSARIum he
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INCReASed ToLeRANCe To WheAT PoWdeR
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The homoeoLogouS RegIoNS oN LoNg AR
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WAyS To ImPRoVe Weed ComPeTITIVe AB
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fhB ReSISTANT modeL VARIeTy of NoRT
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mININg A CoLLeCTIoN of WheAT LANdRA
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IdeNTIfICATIoN of SouRCeS of ReSIST
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ChARACTeRIzATIoN of STem RuST ReSIS
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The 2 nd generation slow-rusters ha
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virus complex present across the fi
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During the same years grain quality
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mAPPINg of AduLT PLANT STem RuST Re
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IdeNTIfICATIoN of geNeTIC ReSISTANC
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APPLyINg mALe STeRILITy medIATed mA
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etween these parameters, indicating
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the field and one in pots placed ou
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ReSeARCh oN INheRITANCe of yeLLoW R
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ReSISTANCe BReedINg AgAINST LeAf SP
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PoWdeRy mILdeW ANd LeAf RuST ReSIST
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oLd CzeCh WheAT CuLTIVARS AS A NeW
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isk will vary depending on the path
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to a number of countries including
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geNeTIC dIVeRSITy of The fuNgAL Whe
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accessions, producing durum wheat w
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ReLATIoNShIPS BeTWeeN fuSARIum heAd
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PoPuLATIoN STRuCTuRe of PuCCINIA TR
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LeAf RuST ReSISTANCe geNeS LR10, LR
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ComBININg WheAT RuST ANd fuSARIum h
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LeAf RuST ReSISTANCe geNe LR67, A T
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STATuS of WheAT PAThoSySTemS fRom 1
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WkS1 (WheAT kINASe START) LImITS gR
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quANTITATIVe TRAIT LoCI foR ReSISTA
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geNeTIC ANALySIS of STem RuST ReSIS
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geNeTIC ImPRoVemeNT of WheAT ReSIST
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ChARACTeRIzATIoN of A NoVeL gLuTeNI
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effeCT of WheAT CuLTIVARS oN VARIAB
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dIVeRSITy of geNeS of ReSISTANCe To
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TAke-ALL ReSISTANCe of euRoPeAN WIN
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dISCoVeRy of TWo quANTITATIVe ReSIS
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Parula had leaf rust severity betwe
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quANTITATIVe PCR AS A TooL foR TILL
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quANTITATIVe TRAIT LoCI ASSoCIATed
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gLoBAL INITIATIVeS foR mANAgemeNT o
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deoxyNIVALeNoL LeVeL IN WheAT gRAIN
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VIRuLeNCe STRuCTuRe of The WheAT ST
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ReLeASe STem RuST ReSISTANT VARIeTI
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AN ASSoCIATIoN geNeTICS APPRoACh To
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cantly greater number of lines (59)
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The INTRoduCTIoN INTo WINTeR WheAT
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effeCTIVe yeLLoW RuST ReSISTANCe ge
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geNeTICS of ReSISTANCe To RACe TTkS
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partially resistant as these genoty
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The moST ImPoRTANT WheAT dISeASeS I
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SPRINg WheAT BReedINg foR LeAf ANd
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CommoN RooT RoT ReACTIoN IN A dIVeR
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hAPLoTyPe ANd ASSoCIATIoN ANALySeS
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IdeNTIfICATIoN of A NeW ReSISTANCe
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mARkeR-ASSISTed SeLeCTIoN foR LeAf
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ReACTIoN of ToLeRANT WheAT geNoTyPe
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that changes in the composition of
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deVeLoPmeNT of PCR-BASed mARkeRS fo
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BIoCoNTRoL of fuSARIum heAd BLIghT
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ImPRoVINg WheAT STRIPe RuST ReSISTA
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BReedINg AduLT PLANT ReSISTANCe To
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improve localisation of QTL positio
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ContRibutions of CRoP Physiology to
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ToWARdS The uNdeRSTANdINg of geNeTI
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SPIke gRoWTh ReguLATINg RATe of deA
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quANTIfyINg hoW WINTeR WheAT CRoPS
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domeSTIC WheAT PRoduCTIoN ANd fuTuR
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for genetic enhancement through int
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IdeNTIfICATIoN of duRum WheAT geNoT
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GPC varied significantly by effect
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geNeTIC VARIABILITy of NITRogeN uSe
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effICIeNCy ANd ChALLeNgeS of PReCIS
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AChIeVemeNTS of WheAT BReedINg AT d
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vs. 7.8 in S1). Grain number explai
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ImPACT of CImmyT PRogRAmS oN WheAT
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gRAIN yIeLd ANd quALITy of WINTeR W
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BReedINg of WheAT IN kyRgyzSTAN ANd
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WheAT fLoReT deVeLoPmeNT IN ReSPoNS
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IdeNTIfyINg NoVeL TRAITS ANd geNeTI
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IdeNTIfICATIoN of TRAITS To ImPRoVe
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geNoTyPe ANd TeSTINg SITe eVALuATIo
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dWARfINg (RhT) ANd PhoToPeRIod INSe
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SeLeCTINg WINTeR WheAT geNoTyPeS fo
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APPLICATIoN of geNoTyPe x eNVIRoNme
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ReSeARCh AdVANCe of The ChoNgqINg-T
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INTeRNAL moRPhoLogICAL ChANgeS of f
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geNeTIC PRogReSS IN WheAT yIeLd ANd
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WheAT BReedINg IN TAJIkISTAN Muminj
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yIeLd ANd Some PhySIoLogICAL ChARAC
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CRoP yIeLd IN duRum WheAT ANd The P
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SySTem APPRoACh To ANALySIS of yIeL
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ChANgeS of SPIke ARChITeCTuRe duRIN
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to that of Sr36 cultivars. The powd
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seed is produced by breeders of the
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effeCTS of SPeCIfIC RhT ANd PPd ALL
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mAIN dIReCTIoNS of AdAPTIVe BReedIN
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PhoToPeRIod SeNSITIVITy IN duRum Wh
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elongatum through cultivar Teewon,
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increase yield above 30% or 9.755t/
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mostly was used to synthesize prote
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geNomIC TeChNoLogIeS ANd ReSouRCeS
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NeW BReedINg TooLS ImPACT CANAdIAN
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geNoTyPIC STRuCTuReS of The CImmyT
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INSeRTIoN SITe-BASed PoLymoRPhISm (
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has been established at the Nationa
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geNomIC ABuNdANCe of VARIouS SImPLe
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STRuCTuRAL ReARRANgemeNTS of WheAT
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geNeRAL STATISTICAL PoWeR of NeSTed
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LoCALIzATIoN of qTLS foR AgRoNomIC
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uSINg mARkeR ASSISTed SeLeCTIoN IN
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PoPuLATIoN STRuCTuRe IN A CoRe CoLL
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was observed. Cultivars that belong
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A QTL was considered to be “stabl
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deVeLoPINg A ReCuRReNT SeLeCTIoN PR
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hIgh effICIeNCy TRANSfoRmATIoN of h
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deTeCTIoN of dNA PoLymoRPhISmS ASSo
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IdeNTIfICATIoN ANd ANALySIS of TAWR
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geNeTIC modIfICATIoN of PhoToSyNThe
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isolated were mapped to the chromos
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stress. Transgenic Arabidopsis plan
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geRmPLASm eNhANCemeNT ANd NeW VARIe
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(174%) than in pedigree schemes (21
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moLeCuLAR CyTogeNeTIC ChARACTeRIzAT
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PhySIoLogICAL ANd BIoChemICAL APPRo
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ChARACTeRIzATIoN of The geNeTIC VAR
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ToWARdS A PhySICAL mAP of SST1, A m
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effICIeNT INCoRPoRATIoN of AduLT PL
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deVeLoPmeNT ANd APPLICATIoN of TRIT
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uSe of hIgh moLeCuLAR WeIghT gLuTeN
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foRmATIoN of geNeTIC VARIABILITy AT
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group were rough with difficult thr
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VALIdATIoN of 86 moLeCuLAR mARkeRS
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genetiC vARiAtion of gli-2 in kAzAk
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of DNA sequence variations sometime
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VARIABILITy of SALINITy ToLeRANCe R
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484
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ImPRoVINg The heALTh BeNefITS of Wh
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hARVeSTPLuS: A gLoBAL effoRT To INC
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deVeLoPmeNT of WheAT geNoTyPeS WITh
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dyNAmIC ChANge of gRAIN SugAR, STAR
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component analysis performed on qua
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associated to the cultivar effect.
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and diversity associated with quali
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levels. It suggests that it is easi
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geNeTIC VARIABILITy of duRum WheAT
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uTILIzATIoN of oLd WheAT VARIeTIeS
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The effeCTS of gPC-B1 oN gRAIN PRoT
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ISoLATIoN of dIffeReNTIALLy exPReSS
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eVALuATIoN meThod foR quALITy ChARA
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in 14 out of 41 specifications (34.
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and no significant effect of Glu-A3
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PSy-A1 ALLeLeS ANd oTheR geNeS CoNT
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mAPPINg qTLS foR LIPoxygeNASe ACTIV
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The mean value of LV at low N rate
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CuRReNT STATuS ANd PeRSPeCTIVeS foR
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INTeRACTIoN effeCTS of eNVIRoNmeNTA
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this study). In terms of gluten str
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Second problem that had to be solve
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dough VISCo-eLASTIC PRoPeRTIeS of R
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gRAIN quALITy of WINTeR WheAT CuLTI
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BReedINg WheAT foR hIgh ToCoL CoNTe
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qTL ANALySIS of PRINCIPAL ANd ePIST
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PRedICTIoN of BAkINg quALITy By SoL
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Ukraine has two geographical-climat
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acid between 16 and 21 mg/g, vanill
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Raweta cv. by 10%. Grains of invest
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for both traits. Besides, group of
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Five pairs of epistatic effects wer
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odies, which could form either by d
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References Lewis S., Bullrich L., K
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BReedINg foR eNhANCed gRAIN-zINC AN
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geNomIC ANd PRoTeomIC STudIeS of gL
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558
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© 2010, N.I. Vavilov Research Inst
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INTERRELATIONSHIPS OF AGRONOMIC CHA
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MULTIVARIATE ANALYSIS OF GENETIC DI
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A METHOD OF ASSESSING SPIKE FERTILI
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IDENTIFICATION OF LMW GLUTENIN SUBU
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8th INTERNATIONAL WHEAT CONFERENCE

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