8th INTERNATIONAL WHEAT CONFERENCE

ImPRoVINg The fRequeNCy of gReeN PLANTLeT
geNeRATIoN uSINg ISoLATed mICRoSPoRe CuLTuRe
(ImC) foR douBLed hAPLoId PRoduCTIoN IN WheAT
ANd TRITICALe
Harpinder Randhawa, Aakash Goyal, Leslie Bihari,
Eric Amundsen and François Eudes
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
E-mail Address of presenting author: harpinder.randhawa@agr.gc.ca
Doubled haploid techniques enable plant breeders to speed up the production of homozygous
lines for cultivar development. Earlier in 1990’s, doubled haploids have been
predominantly produced either by anther culture or ovule culture. In recent years new
techniques either Isolated Microspore Culture (IMC) or wheat-maize pollination methods
are being used. IMC method has advantages over the wheat-maize pollination
method as IMC has the potential to produce hundreds of haploid plants per culture as
compare to one haploid plant per floret using wheat-maize pollination method. Further,
IMC method has the higher rate of spontaneous chromosome doubling of haploid plants.
However, the production of doubled haploids has always been a challenge in case of cereals
with higher number of albino plants regenerated. The effect of different antioxidants
on embryo formation and frequency of green plants regeneration through IMC for wheat
and triticale was studied on four spring wheat and one triticale lines. The results indicate
that the higher frequency of green plant regeneration was obtained with the application
of 10mM NtBHA in wheat, however, in triticale higher green plants was obtained in the
control. The synergistic effect of all the antioxidants was negative for both wheat and
triticale. The effect of antioxidants on embryo formation and of green plants regeneration
was also depended on the genotype.
465