8th INTERNATIONAL WHEAT CONFERENCE

No chromosome rearrangements were detected between wheat and Ae. biuncialis chromosomes
in this addition line using GISH. Line No. 1575/08 had 42 wheat chromosomes
and a 2U b chromosome pair. Progeny of the line No. 33/01 was identified as a 6U b monosomic
addition. Line No. 1564/08 carried an extra satellited Ae. biuncialis chromosome
pair, which was small-sized (possibly it is a deletion chromosome). Line No. 1583/09
carried 5M b , 6M b and 7M b chromosomes, while line No. 1589/09 had 7U b , 1U b and 3U b
chromosomes in wheat background. It is planned to analyse these lines using GISH to detect
chromosome rearrangements. The morphological traits and resistance of these lines
will be investigated in the nursery. The aim of the experiments was thus to select U and
M genome-specific SSR markers. The first task was to select markers that gave a polymorphic
band on Ae. biuncialis compared with wheat, after which these markers were selected
for U and M genome specificity on wheat–Ae. biuncialis and wheat–Ae. geniculata
(2n=4x=28, U g U g M g M g ) addition lines. In this study 108 wheat SSR markers were tested
on the wheat and on Ae. biuncialis. It was found that 78.70% of the wheat microsatellite
markers tested gave polymorphic or non-polymorphic PCR products on Ae. biuncialis.
Fifty of the 108 markers (46.29%) did not exhibit polymorphism between wheat and Ae.
biuncialis. For 23 of the markers (21.29%) bands were obtained on wheat, while no PCR
product was observed on Ae. biuncialis. A further 35 SSR markers (32.40%) proved to be
polymorphic, i.e. PCR products were obtained on both wheat and Ae. biuncialis, but the
fragment lengths differed. Of the 35 polymorphic primer pairs that gave products on Ae.
biuncialis, three (8.57%) gave specific PCR products on the wheat-Ae. biuncialis or the
wheat–Ae. geniculata addition lines. It can be assumed that the remaining 32 polymorphic
markers were not located on Ae. biuncialis chromosomes 2M b , 3M b , 7M b , 3U b or 5U b ,
but on those for which no addition lines are as yet available. The markers Xgwm44 and
Xgdm61 gave specific PCR products on the 2M b and 3M b wheat–Ae. biuncialis addition
lines, but not on the 2M g addition line of Ae. geniculata. A specific band was observed on
the 7U g wheat–Ae. geniculata addition line for the Xbarc184 primer.
This work was financially supported by the Hungarian National Scientific Research Fund,
No. PD75450, the Generation Challenge Programme (CGIAR GCP SP3 G4007.23), the
AGRISAFE Project (No. 203288 EU-FP7-REGPOT 2007-1) and the János Bolyai Research
Scholarship of the Hungarian Academy of Sciences.
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