8th INTERNATIONAL WHEAT CONFERENCE

VALIdATIoN of 86 moLeCuLAR mARkeRS
ANd IdeNTIfICATIoN of 56 geNeS, ALLeLeS ANd qTLS
IN ChINeSe WheAT CuLTIVARS
Guozhong Sun 1, 2 , Guangxia You 1 , Fei Yuan 1 , Jingyan Sun 2 ,
Haibo Wang 2 , Shihe Xiao 1
1 Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, P.R.
China
2 Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Science,
Shijiazhuang 050051, P.R. China)
E-mail Address of presenting author: sungz2001@yahoo.com.cn
In order to improve the wheat breeding efficiency through marker assisted selection
(MAS), the validation of 86 molecular markers previously reported was analyzed,
including powdery mildew resistance (12), rust resistance (26), fusarium head blight
resistance (5), sharp eyespot resistance (3), barley yellow dwarf disease resistance (1),
response to vernalization (6), response to photoperiod (2), plant height (7) and grain
quality (24). Moreover, the distribution of 76 markers associated with 56 different genes,
alleles and QTLs in a total of 75 Chinese wheat cultivars collected from ten wheat growing
provinces was identified. The results indicated that 9 markers were non-specific and
unsuitable for genotype identification, 31 markers were moderately specific and worthy
of further genetic study, and 46 markers were highly specific and very suitable for MAS.
11 genes, such as Yr5, Yr10, Yr15, Lr34, Lr35, Wx-4A and Wx-7D, were not found in
the 75 wheat cultivars; the distribution ratio of 23 genes was lower than 10%, including
Pm21, Pm4a, Pm4b, Pm13, Pm24, YrCH42, Yr26, SC-W37U, Bx14, Vrn-B1 and Ppd-
D1b. The usefulness of current information in wheat breeding is discussed.
Key words: wheat, molecular marker, molecular identification
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