8th INTERNATIONAL WHEAT CONFERENCE

ChARACTeRIzATIoN of hIgh- ANd LoW-moLeCuLAR-
WeIghT gLuTeNIN SuBuNIT geNeS IN ChINeSe WINTeR
WheAT CuLTIVARS ANd AdVANCed LINeS uSINg ALLeLe-
SPeCIfIC mARkeRS ANd SdS-PAge
F. P. Yang 1, 2 , L. H. Wang 1 , J. W. Wang 1 , X. Y. He 1 ,
W. X. Yang 2 , X. C. Xia 1 , and Z. H. He 1
1 Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility
for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences
(CAAS), 12 Zhongguancun South Street, Beijing 100081, China.
2 Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070,
China.
E-mail Address of presenting author: yfp1023@163.com
Wheat end-use product quality is highly influenced by the composition and quantity
of high- and lowmolecular-weight glutenin subunits (HMW-GS and LMW-GS). In the
present study, 224 Chinese wheat cultivars and advanced lines were characterised for the
HMW-GS and LMW-GS with allele-specific PCR markers and sodium-dodecylsulfate
polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that 56 cultivars
(25.0%) carried the allele Glu-D1-1d (Dx5), while 80 cultivars (35.7%) with the allele Glu-
B1-2a (By8) produced a 527-bp specific band. Fourteen genotypes (6.3%) with the allele
Glu-B1e (Bx20) yielded a 701-bp amplicon with the marker Mar and a 753-bp specific
PCR fragment with the marker ZSBy9aF1/R3. Glu-B1h (Bx14+By15) was present in only
1 genotype, and 2 cultivars contained the allele Glu-B1f (Bx13+By16) identified with the
marker ZSBy9F2/R2. Four genotypes (1.8%) with the allele Glu-B1-1d (Bx6) gave 695-bp
and 830-bp bands, and 5 genotypes (2.2%) with the allele Glu-B1i Bx17+By18) amplified
a 659-bp fragment using the marker Bx. One hundred and six cultivars (47.3%) had the
allele Glu-B1-2b (By9), amplifying a 663-bp fragment with the marker ZSBy9aF1/R3; 34
genotypes (15.8%) contained the allele Glu-B3d, generating a 662-bp PCR fragment with
the marker gluB3d. Fifteen cultivars (7.0%) with the allele Glu-B3b yielded 1570-bp and
750-bp PCR amplicons with the markers gluB3b and gluB3bef, respectively. The allele
Glu-B3h was found in 7 cultivars, generating a 1022-bp PCR fragment with the marker
gluB3h. The genotypes detected by SDS-PAGE were mostly consistent with those identified
by molecular markers, confirming the utility of the molecular markers. The information
for the HMW-GS and LMW-GS in Chinese wheat cultivars will be useful in wheat
breeding programs.
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