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historical perspectives: from the hasmoneans to bar kokhba in light ...

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THE GENETIC SIGNATURE OF<br />

THE DEAD SEA SCROLLS<br />

GILA KAHILA BAR-GAL,' CHARLES GREENBLATT,'<br />

SCOTT R. WOODWARD , 2 MAGEN BROSHI, S AND PATRICIA SMITH*<br />

Introduction<br />

The Dead Sea Scrolls are unique early records of our cultural heritage.<br />

Some of <strong>the</strong> scrolls were nearly complete when found but <strong>the</strong><br />

majority were greatly fragmented. Some fragments could be pieced<br />

<strong>to</strong>ge<strong>the</strong>r by match<strong>in</strong>g text patterns, scribal characteristics, <strong>in</strong>k, and<br />

fac<strong>to</strong>rs such as physical damage (Stegemann, 1992), but many o<strong>the</strong>rs<br />

are still unmatched. New technologies developed <strong>in</strong> forensic science<br />

and anthropology have demonstrated that it is possible <strong>to</strong> recover<br />

DNA <strong>from</strong> archeological specimens, <strong>in</strong>clud<strong>in</strong>g parchment (Woodward<br />

et al., 1996). This new technology may help us <strong>to</strong> match <strong>the</strong>se fragments,<br />

as well as <strong>to</strong> reveal more about <strong>the</strong> choice of animals whose<br />

sk<strong>in</strong>s were used for writ<strong>in</strong>g <strong>the</strong>se texts.<br />

DNA conta<strong>in</strong>s <strong>the</strong> genetic <strong>in</strong>formation of an organism. This <strong>in</strong>formation<br />

is coded <strong>in</strong> genes and is capable of self replication. DNA is<br />

found <strong>in</strong> both <strong>the</strong> nucleus and cellular organelles—<strong>the</strong> mi<strong>to</strong>chondri<br />

a—which are found <strong>in</strong> multiple copies <strong>in</strong> <strong>the</strong> cell. In this research<br />

we analyzed <strong>the</strong> DNA of two different regions <strong>in</strong> <strong>the</strong> mi<strong>to</strong>chondria,<br />

<strong>the</strong> cy<strong>to</strong>chrome b and <strong>the</strong> mi<strong>to</strong>chondrial control region (D-loop).<br />

Each of <strong>the</strong>m is about 1,100 nucleotides <strong>in</strong> length. The nucleotides<br />

are <strong>the</strong> molecules that compose <strong>the</strong> DNA. The cy<strong>to</strong>chrome b gene<br />

is species-specific while <strong>the</strong> D-loop region can identify species, <strong>in</strong>dividuals<br />

and closely related <strong>in</strong>dividuals such as would be seen <strong>in</strong> a<br />

herd or flock (Irw<strong>in</strong>, 1991).<br />

The DNA recovered <strong>from</strong> ancient specimens is fragmented <strong>in</strong><br />

pieces several hundred nucleotides long. We can devise means of<br />

1 Sanford F. Kuv<strong>in</strong> Center for <strong>the</strong> Study of Infectious and Tropical Diseases,<br />

Hadassah Medical School, Hebrew University of Jerusalem.<br />

2 Department of Microbiology, Brigham Young University.<br />

3 The Israel Museum, Jerusalem.<br />

* Labora<strong>to</strong>ry of Bioanthropology and Ancient DNA, Faculty of Dental Medic<strong>in</strong>e,<br />

Hebrew University of Jerusalem.

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