Urinalysis - The Carter Center

forget to raise the condenser and opening of the diaphragm
when you change the objective in to the high power (40x).
Under high power objective also you should have to look for
a minimum of 10-15 fields).
k) Then report what you get under10 x (low power) and 40 x
(high power) on the laboratory request form of the patient.
For determination of cellular elements, casts, etc, the
number of elements seen under at least 10 fields should be
counted and the average of this number is used for report
value. Other elements such as parasites are usually
reported as well.
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5.2. Source of Errors in the Microscopic Examination of
Urine
Possible errors that may encounter during microscopical examination of
urine include:
• Drying of the specimen on the slide.
• During trial of observing 2 specimens in a single slide by putting at
each side of slide, (mix up of the specimens).
• If the supernatant fluid after centrifugation is not poured off properly,
that is if some drop is left in the tube, it may decrease concentration
of urine sediments and false result may be reported
• If the whole sediment with supernatant is discarded during inverting
down the tube for long period, the whole sediments will be discarded
and so again false negative result will be reported.