Urinalysis - The Carter Center
Urinalysis - The Carter Center
Urinalysis - The Carter Center
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33<br />
Calibration<br />
To obtain the correct specific gravity readings in urine, the urinometer<br />
must be weighted to read exactly 1.000 in distilled water. <strong>The</strong> reading<br />
on the urinometer scale should be exactly 1.000. If it is not, a correction<br />
must be applied to all values obtained for urine specimens with the<br />
urinometer. For Example, Suppose the urinometer reads 1.002 in<br />
distilled water. <strong>The</strong> specific gravity of water is 1.000. <strong>The</strong>refore the<br />
urinometer correction is 0.002 must be subtracted from the subsequent<br />
relative specific gravity. If a urine specimen has an apparent specific<br />
gravity of 0.037, this value minus 0.002 results in the corrected specific<br />
gravity of 1.035 for the urine specimen.<br />
Temperature Correction<br />
<strong>The</strong> specific gravity of a solution is dependent on temperature. Most<br />
urinometers are calibrated for use at 15 o C. For each 3 o C difference<br />
0.001 must be added if above, or subtracted if below than the calibration<br />
temperature. For example, if the specific gravity of the urine is 1.022 at<br />
23 o C, and the urinometer has been calibrated at 20 o C, the correct<br />
reading is 1.022+0.001= 1.023.However, significant error will result if<br />
the reading is taken on the urine specimen that has been refrigerated.<br />
Instead of applying this correction, the urine specimen should be allowed<br />
to warm up to room temperature before its specific gravity is determined.<br />
Correction for abnormal Dissolved Substances<br />
<strong>The</strong> specific gravity increases by 0.004 for every 1% glucose in urine<br />
and 0.003 for every 1% protein in solution. <strong>The</strong>refore subtract 0.004 from<br />
the specific gravity reading for every 1% glucose in urine. And subtract<br />
0.003 from the specific gravity reading for every 1% protein in the urine.<br />
It is not usual however for the Laboratory Technician to correct specific<br />
gravity readings for the presence of sugar or protein when laboratory<br />
results are reported. Instead, the clinician will be aware that the specific