Urinalysis - The Carter Center

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67 4. A blue to green color indicates a positive reaction. 5. Report as positive or negative. Prparation of Fouchet's Reagent (See Reagent Number 9 ) B. Barium chloride Filter Paper Method It is the modification of Harrison's test. The barium chloride is supplied on thick filter paper that has been soaked in a saturated solution of barium chloride. Principle The principle of the test is the same as Harrison's (Fouchet's) Test. Procedure 1. Hold a strip of barium chloride paper perpendicularly in the urine for a few seconds 2. Place one or two drops of Fouchet's Reagent on the saturated area. 3. Look for the appearance of a green color, which constitutes a positive reaction. 4. Report as positive (formation of a green color) or negative (formation of any color except green, or no color formation). Preparation of Barium Chloride Filter Paper( See Reagent Number 3) Sensitivity Harrison's Test is so sensitive that it detects as small as 0.1 - 0.2 mg of bilirubin in 100ml of urine. C. Diazotization Tests for Bilirubin The tablet and reagent strip tests for bilirubin are based on the coupling of bilirubin with a diazonium salt in an acid medium to form azobilirubin, which gives a blue or purple color.
68 1. Icotest Tablet Test The Ictotest tablet contains nitrobenzine diazonium, p-toluene sulfonate (bilazo), sulfosalicylic acid, and sodium bicarbonate. The mats are absorbent asbestos cellulose. Procedure 1. Place five drops of urine on either side of the special test mat supplied with the reagent tablets. 2. Place the tablet in the center of the moistened area. 3. Flow two drops of water on the tablet. 4. Observe the mat around the tablet for the appearance of a blue to purple color within 30 seconds. 5. Report the results as positive or negative according to the following criteria. Negative: The mat shows no blue or purple within 30 seconds. Ignore any color that forms after 30 seconds or a slight pink or red that may appear. Positive: The mat around the tablet turns blue or purple within 30 seconds. Ignore any color change on the tablet itself. Sensitivity: Ictotest detects 0.1mg of bilirubin in 100ml of urine. 2. Reagent Strip Tests for Bilirubin (Ex. Multistix) Principle These tests for bilirubin are available only on multiple-reagent strips in conjugation with other tests. They are diazotization tests and are analogous to the Icotest tablet test. The test area for bilirubin on
Page 1 and 2:
LECTURE NOTES For Medical Laborator
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Preface i Books on Medical history
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Contents iii Preface Aknowledgement
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v List of Table Table 1 Methods of
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Abbreviations vii ADH : Antidiureti
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ix This lecture note is prepared fo
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Components of the renal system are
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4 collecting tubule. A number of th
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- Electrolytes - Cast parasites and
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Exercise 1. 8 Answer the Following
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10 plastic tube with a plastic snap
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12 24- Hour specimen - a specimen o
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• Distruction of glucose by bacte
Page 27 and 28: 16 2.3 Type of Examination in Routi
Page 29 and 30: 18 CHAPTER THREE Physical Examinati
Page 31 and 32: • Myxedema • Some type of tubul
Page 33 and 34: metabolic conditions, the color and
Page 35 and 36: - By letting it to stand for more t
Page 37 and 38: • Degree of cloudiness of the uri
Page 39 and 40: - Look for color change of red litm
Page 41 and 42: tubules of the nephron, depending o
Page 43 and 44: Specimen: It should be the first ur
Page 45 and 46: gravity is elevated because of the
Page 47 and 48: 36 Exercises Say True or False 1. U
Page 49 and 50: 38 constituents of urine. 4.1 Deter
Page 51 and 52: 40 Causes of Glycosuria • Physiol
Page 53 and 54: specimen and the results are graded
Page 55 and 56: unnecessary.The reaction for clinit
Page 57 and 58: 46 The overall reaction is : Glucos
Page 59 and 60: 2. Dip part of the tape into the ur
Page 61 and 62: metabolism or absorption, the body
Page 63 and 64: 4. If acetone or acetoacetate is pr
Page 65 and 66: immediately. For this reason, Gerha
Page 67 and 68: contains only traces of protein, in
Page 69 and 70: It requires large volumes ( 0.5 to
Page 71 and 72: due to urates, uric acid urea and b
Page 73 and 74: 62 Preparation of Acetic Acid Reage
Page 75 and 76: urine contains more than 100mg/dl p
Page 77: cell disease (hepatocellular diseas
Page 81 and 82: characteristic color with the other
Page 83 and 84: 72 Sensitivity Urobilistix detects
Page 85 and 86: Hemoglobinuria may occur with sever
Page 87 and 88: Since all these tests are based on
Page 89 and 90: peroxidase activity) catalyzes the
Page 91 and 92: 80 4.9 Determination of Nitrite Int
Page 93 and 94: The pink color is therefore related
Page 95 and 96: - Pancreatic insufficiency. - Intes
Page 97 and 98: 86 A. Ferric Chloride Test Principl
Page 99 and 100: 88 CHAPTER FIVE Microscopic Examina
Page 101 and 102: • If it is difficult to deliver w
Page 103 and 104: 92 5.3 Urinary Sediments Classifica
Page 105 and 106: Red blood cells are some what round
Page 107 and 108: EPITHELIAL CELLS • Normally few e
Page 109 and 110: 98 CASTS • Formed by precipitatio
Page 111 and 112: 100 c. Waxy Casts (Renal Failure Ca
Page 113 and 114: 102 • Always the condenser should
Page 115 and 116: 104 • Large, measuring 275-399 x
Page 117 and 118: 106 Report of the Result The bacter
Page 119 and 120: 108 Some drug crystals in the urine
Page 121 and 122: 110 - Have various tiny squarish, b
Page 123 and 124: 112 - Size is about 20 μm. - Often
Page 125 and 126: 114 They can be confirmed by logos
Page 127 and 128: 116 Nucleus Cytoplasm Squameous epi
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118 Reagent No.5 Bleach for Preserv
Page 131 and 132:
120 Reagent No.16 Saturated Sodium
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122 III. Table 4: Correct and Incor
Page 135 and 136:
124 Glomeurlar Filtration Rate: The
Page 137 and 138:
126 Urinometer: A small glass instr
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Urinalysis - The Carter Center

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