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Urinalysis - The Carter Center

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118<br />

Reagent No.5 Bleach for Preservation of S.Hematobium egg in<br />

urine<br />

S. hematobium. egg preserved by adding 1ml (1-% v/v).<br />

Domestic bleach in every 10 ml urine.<br />

Reagent No.6 Boric Acid Preservative (1-%w/v) 10 gm/l<br />

Boric acid --------------10gm.<br />

Dissolve in 1000ml distilled water.<br />

Reagent No.7 Ehrlich's Reagent for Uroblinogen<br />

To make 200ml<br />

para-dimethylaminobenzaldehyde --------------------- 4 gm<br />

HCl concentrated ----------------------------------------- 40 ml<br />

Distilled H 2 O --------------------------------------------- 160ml<br />

a. Weigh the para-dimethylaminobenzaldeyde and<br />

transfer it to clean, leak proof bottle.<br />

b. Measure the water and add to chemical and mix<br />

c. Add conc. HCl and mix well.<br />

d. Label the bottle and mark, as it is corrosive.<br />

Store at room temperature the reagent is stable<br />

for several weeks.<br />

Reagent No. 8 10 % Ferric Chloride Reagent<br />

Weigh 10 g of ferric chloride and transfer to a 100-ml volumetric flask.<br />

Dissolve and dilute to 100ml volume with water and mix.<br />

Reagent No. 9 Fouchet’s Reagent<br />

Trichloroacetic acid ---------------------- 25gm<br />

Distilled H 2 O ------------------------------100ml<br />

10% Ferric chloride (FeCl 3 ) -------------10ml<br />

Mix well.

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