Urinalysis - The Carter Center

Contents of tablet
The acetest tablet contains Sodium Nitroprusside (Nitroferricyanide),
Glycine, and a strongly Alkaline buffer.
D. Acetone Powder Test
Procedure
1. Place a small amount of acetone powder on a clean sheet of
paper.
2. Add three or four drops of urine.
3. A purple color is a positive test for acetone and diacetic acid.
Preparation of Acetone Powder Reagent (See Reagent Number 2)
E. Ketostix, Rreagent Strip Test
Procedure
1. Dip test- end of the strip in urine
2. At 15 seconds compare the color of dipped- end with the color
chart.
3. Report as indicated by the color chart.
Contents of Reagent Strip
It contains Sodium Nitroprusside, Glycine, and a strongly Alkaline buffer.
In alkaline medium, acetoacetic acid (diacetic acid) and acetone react
with nitroprusside in the presence of glycine to form a purple complex.
Gerhardt's Test for Acetoacetate (Diacetic Acid)
Gerhardt's test is specific for acetoacetate (diacetic acid); however it is
capable of detecting only large amounts of acetoacetate. The test has
been used as a means of determining the severity of ketosis. A positive
result indicates severe ketosis, and treatment must be started
53