Urinalysis - The Carter Center

urine contains more than 100mg/dl protein, dilute the urine and
repeat the test.
B. Esbach’s Test
1. If a 24 hour urine collection is used, first, measure the total
volume; then filter some of the urine. The urine must be clear.
2. Do qualitative protein test, Robert’s or strip test.
- if the urine is +3, made 1:5 dilution.
- if the urine is +4 make 1:10 dilution.
- if the urine is trace, +1 or +2 non dilution is needed.
- if the urine is negative, a quantitative test is not done.
3. Test measure the pH of the urine. It should be acidic. If not, add
10 % acetic acid.
4. Add pumice powder to the 0.5 mark of the Esbach’s tube.
5. Add urine to the “U” mark.
6. Add Esbach’s reagent to the “R” mark.
7. Mix slowly by inversion, 10 times.
8. Wait for 30 minutes. Read the highest of the column. Do not
subtract the amount of the pumice.
9. The result is now in gram per liter of protein in the urine. If the
urine has been diluted, multiply by the dilution factor, calculate,
and record the g % and the g / 24 hrs
Final report should include total volume.
The following formula is used to calculate the amount of urinary
protein excreted in 24 hrs.
64
g/24 hr = total volume x g/l
1000