Urinalysis - The Carter Center

of moisture and must be kept in a cool, dry place, away from direct heat
and sunlight.
Sensitivity
Clinitest reagent tablets will detect as little as 250mg of sugar in 100ml of
urine.
Specific ( Enzymatic ) Tests
Enzymatic tests are specific tests for glucose. They are reagent strips
(dipsticks ), which are impregnated with enzymes glucose oxidases.
Glucose oxidase catalyzes only the oxidation glucose to gluconic acid
and hydrogen peroxide. The principle of all enzymatic, which is based
on the uses of glucose oxidase, is the same. They differ only on the
uses of different type of chromogen (a color indicator ).
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A. Clinistix Reagent Strip Test
Principle
This is a specific test for glucose based on the use of the enzyme
glucose oxidase, which is impregnated on a dip strip. In this test glucose
oxidase oxidizes glucose to gluconic acid and at the same time
reduces atmospheric oxygen to hydrogen peroxide. The hydrogen
peroxide formed , in the presence of the enzyme peroxidase,
oxidizes the reduced form of o-toluidine( a chromogen ) to oxidized
form of the indicator, wich produces a color change proportional to
the amount of glucose in the urine. Other sugars are not substrates
for the enzyme do not react with this test.
A positive reaction is seen as a change of color from red to blue,
depending on the amount of glucose present in the urine.