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Urinalysis - The Carter Center

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peroxidase activity) catalyzes the oxidation of orthotolidine by the<br />

peroxide. <strong>The</strong> oxidized orthotolidine is blue.<br />

Procedure:<br />

1. Follow the manufacturers directions and precautions.<br />

2. Dip the test end of strip into a well-mixed specimen of urine and<br />

remove immediately.<br />

3. After 30 seconds compare the test area with the color chart<br />

provided.<br />

4. Report as indicated on the color chart.<br />

Composition of the strip<br />

78<br />

<strong>The</strong> test area is impregnated with orthotolidine, cumene hydroperoxide<br />

and citrate buffer.<br />

4.8 Determination of Urinary Calcium<br />

Introduction<br />

<strong>The</strong> bulk of calcium ions (Ca++) discharged by body is excreted in the<br />

stool. However, there is small quantity of calcium that is normally<br />

excreted urine. But it may increase depending up on the quantity of<br />

dietary calcium ingested. <strong>The</strong> 24 hour test is most often ordered to<br />

determine the function of the parathyroid gland, which maintains a<br />

balance between calcium and phosphorous by means of parathyroid<br />

hormone. Hyperparathyrpidism is a generalized disorder of calcium,<br />

phosphate and bone that results from increased secretion of parathyroid<br />

hormones and an increased excretion of urinary calcium. In<br />

hypoparathyrodism the urinary calcium is decreased.<br />

Interfering Factors<br />

1. False positive<br />

- High sodium and magnesium intake.

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