Urinalysis - The Carter Center

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They can be confirmed
by logos iodine.
* To minimize the above mentioned contaminants and artifacts
- Don't use dirty containers, slides and cover slides.
- Don't let urine specimen to open-air.
- Avoid contamination of urine with fats and oils.
- Avoid the drying of sediments.
Methods for Examining Urine Sediments
A. Unstained Urine Sediment
1. Bright field microscopy of the unstained urine sediment
Traditionally, the urinary sediment has been examined microscopically
by placing a drop of urine sediment on a microscopic slide, cover with
cover slide and observing the preparation with the lower and high power,
objective of the bright field microscope.
When the sediment is examined under the bright field microscope,
correct light adjustment is essential, and the light must be sufficiently
reduced, by the correct positioning of the condenser and the iris
diaphragm to give contrast between the unstained structures and the
back ground liquid.
2. Phase Contrasts (PC)
P.C. illumination is useful in the examination of unstained urinary
sediment, particularly for translucent elements such as hyaline casts
and mucus threads, which have a refractive index similar to that of urine
in which they are suspended. Phase contrast has the advantage of
hardening the outlines even the most ephemeral formed elements.
B. Stained Preparation
Cellular detail is best seen with stained preparation.