Urinalysis - The Carter Center
Urinalysis - The Carter Center
Urinalysis - The Carter Center
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72<br />
Sensitivity<br />
Urobilistix detects 0.1 mg in 100 ml of urine (0.1 Ehrlich units in 100 ml)<br />
4.6 Test For Urobilin<br />
Urobilin is an oxidation product of urobilinogen. Urobilin is colored and<br />
urobilinogen is colorless. Both compounds have the same clinical<br />
significance when present in urine; however, they undergo different<br />
chemical reactions.<br />
Schilesinger's Test for Urobilin<br />
Principle<br />
When zinc acetate reacts with urobilin it produces a green fluorescence.<br />
Procedure<br />
1. Mix equal parts (10 ml each) of urine and alcoholic solution of zinc<br />
acetate in a test tube. Mix and filter the mixture.<br />
2. Take 10 ml of the filtrate and add 2 drops of Lugol's solution. Mix<br />
by inversion.<br />
3. Examine the solution for green fluorescence by viewing the tube<br />
from above as it is passed through the direct rays of a fairly strong<br />
light (sunlight or wood's light).<br />
4. Report as positive or negative. Urobilin produce a green<br />
fluorescence while<br />
porphrins produce red fluorescence.<br />
Preparation of Alcoholic Solution of Zinc Acetate (See Reagent<br />
Number 13)
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