Urinalysis - The Carter Center

Diastix is incorporated in the glucose test area in various other multiplereagent
strips produced by the manufacturer, Ames Co. these other
tests include: Combistix, Ketodiastix, Labstix, Multistix, Uristix and the
like.
Procedure
Follow the directions supplied with the reagent strip.
1. Dip the reagent area of the strip briefly into the specimen.
2. Compare the test area with the colour chart after 10 seconds to
see whether the reaction is positive or negative for glucose.
3. Compare the test area with the color chart at 30 seconds, for a
semiquantitative result, and report the results as indicated on the
chart.
Sensitivity
Diastix reagent strip detects as little as 100mg of glucose in 100 ml of
urine.
4.2 Determination of Ketone Bodies
Introduction
Ketone bodies, also called Ketones, are a group of three related
substances such as, acetone, acetoacetate (acetoacetic acid or diacetic
acid), and β -hydroxybutyrate (β -hydroxybutyric acid).
Ketone bodies are normal products of fat metabolism.They are normally
not detectable in the blood or urine. In normal metabolism, fat is broken
down in the tissues to glycerol and fatty acids. The free fatty acids are
transported by the plasma albumin to the liver where they are broken
down to acetyl coenzyme A (acetyl Co-A) molecules. These condense
with oxaloacetate in the Krebs cycle to produce citrate. The citrate is
then oxidized to produce heat and energy. Whenever there is
inadequate carbohydrate in the diet or a defect in carbohydrate
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