Urinalysis - The Carter Center

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Reagent No. 10
10% Hydrochloric Acid (HCl) Reagent
Measure 10 ml HCl and dissolve in 90ml-distilled water.
Reagent No. 11 Robert's Reagent for Protein
To 1 lit of distilled water add magnesium sulphate (MgSO 4 )
with stirring until no more to dissolve.
Add 200-ml concentrated HN0 3 & mix.
Reagent No.12 Sulkowitch Reagent for Calcium
Weigh 4 gm Ammonium Oxalate and dissolved in 100 ml
distilled water.
Reagent No.13 Alcoholic Solution of Zinc Acetate for Urobilin
Place 100 ml of ethyl alcohol in a beaker.
Add Zinc Acetate with string until no more goes into solution.
Weigh 5 gm of Iodine and 10 gm of KI.
Transfer all reagents to a brown bottle.
Reagent No.14 Sulphosalicylic Acid Reagent ( 20% W/V)
Sulphosalcylic acid ----------------------- 200 gm
Dilute to 1 lit. Volume with distilled H 2 O.
Reagent No.15 Sodiumnitroprusside Rreagent
Weigh 10 gm of Sodium Nitroprusside (Nitroferricyanide).
Transfer to a flask containing 95ml of water and 2ml of
concentrated H 2 SO 4.
Mix and store in a brown bottle.