Urinalysis - The Carter Center

Multistix and other Ames Co. reagent strip products is impregnated with
2,4-dichloro-aniline diazonium salt.
The reagent strip tests for bilirubin are difficult to read and the color
formed after reaction with urine must be carefully compared with color
chart supplied by the manufacturer.
Procedure
1. Dip the reagent strip briefly into the urine specimen.
2. Remove excess urine by tapping the edge of the strip against the
rim of the urine Container. Hold the strip in a horizontal position to
prevent mixing of chemicals from adjacent reagent areas.
3. Compare the test areas for bilirubin closely with the color chart
supplied by the manufacturer. Multistix should be read 20
seconds after dipping.
4. Report the results as positive or negative for bilirubin.
Sensitivity
Multistix detects 0.2-0.4 mg of bilirubin in 100ml of urine.
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4.5 Determination of Urobilinogen
Introduction
In the intestine, most of the bilirubin is converted to urobilinogen or
stercobilinogen by the action of certain bacteria that make up the
intestinal flora. Approximately half of the urobilinogen formed in the
intestine is absorbed into the portal blood circulation and returned to the
liver. In the liver most of the urobilinogen is excreted into the bile once
again and returned to the intestine.
A very small amount of urobilinogen about 1 percent of the formed
urobilinogen is excreted from the body in the urine as urobilinogen or
can be also converted into urobilin, which gives the urine its