Urinalysis - The Carter Center
Urinalysis - The Carter Center
Urinalysis - The Carter Center
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4. If acetone or acetoacetate is present, a red to purple color will<br />
develop at the line of contact. <strong>The</strong> color may not appear for 10-15<br />
minutes. Disregard any brown or orange colors.<br />
5. Report the test as positive or negative.<br />
52<br />
Note: Urine collected after a big meal may give a purplish color within<br />
30 seconds but it fades within 3-4 minutes. This is not a positive<br />
test.<br />
Preparation of Sodium Nitroprusside Reagent (See Reagent Number15)<br />
B. Lang's Test for Acetone and Acetoacetate<br />
Procedure<br />
1. Pour about 5 ml of fresh urine into a test tube.<br />
2. Add 5 drops of glacial acetic acid and a few drops of saturated<br />
solution of sodium nitroprusside mix.<br />
3. Slowly overlay with ammonium hydroxide (28%, full strength)<br />
4. If acetone is present, a purple or reddish purple ring will appear at<br />
the meeting place of the two liquids.<br />
5. Report the test as positive or negative.<br />
Preparation of Saturated Sodium Nitroprusside (See Reagent Number<br />
16)<br />
C. Acetest Tablet Test<br />
Procedure<br />
1. Place an acetest tablet on a piece of white paper.<br />
2. Place a drop of urine on the tablet.<br />
3. If acetone or diacetic acid is present, a purple color will develop<br />
within 30 seconds. (compare with the color chart that comes with<br />
the reagent).<br />
4. Report the test as positive or negative.
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