Status of medicinal and aromatic plants in - Inia

PRESENTED PAPERS 149
Table 2. Plant material, isolation protocols and resulting PCR reaction: (+) = successful amplification,
(o) = partly successful amplification, (-) = unsuccessful amplification
Plant material Isolation protocol PCR reaction
Fresh (frozen) leaves, collected in autumn
Protocol Liber with modifications,
CTAB method
(-)
Fresh leaves, collected in May Dneasy Plant Mini Kit (Qiagen) (-)
Protocol Liber (-)
Leaves from greenhouse
SDS buffer (-)
Qiagen Kit (+)
- fresh Qiagen Kit, SDS buffer (+)
In vitro - frozen (-)
grown - lyophilized (o)
Qiagen Kit
plants - dried at 40°C (+)
- treated with hexan and methanol
(o)
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