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2012 Ocean Ranger Guidebook Revision 3-7-12 - Alaska ...

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2/25/<strong>20<strong>12</strong></strong> CPVEC <strong>Ocean</strong> <strong>Ranger</strong> Program<br />

<strong>20<strong>12</strong></strong> <strong>Ocean</strong> <strong>Ranger</strong> <strong>Guidebook</strong> 3-7-<strong>12</strong><br />

Reasons for not sampling: If vessel officials object or do not want to cooperate in the sampling<br />

event, report this to DEC and terminate the sample. Do not sample if conditions aboard the<br />

vessel are not safe.<br />

Field Records: Use field record form or if this is not available please take notes. Information must<br />

include the vessel name, location with latitude and longitude, sample port used, discharge type<br />

(blackwater, graywater, mixed), discharge status, and who assisted and/or observed the sample<br />

collection. Comments must be included on the form such as why the sample was taken. In the field<br />

notes record the chlorine measurements, pH, and temperature along with time sample was taken. Place<br />

field notes in top lid compartment of cooler before having lab or DEC pick up sample. If possible take a<br />

photograph of the sample port. Photographs should include identifying information- like time, place- for<br />

example include filed notes in photograph.<br />

Custody: <strong>Ocean</strong> <strong>Ranger</strong> to stay with the box until it is picked up and custody form is singed and<br />

completed. If vessel officials object or do not want to cooperate in the sampling event, report this to<br />

DEC and terminate the sampling process.<br />

Procedures:<br />

Pre-sample:<br />

1) Get ice: Partially fill cooler with combination of ice and water to make ice water slurry. Leave<br />

enough room for full sample bottles.<br />

2) Inflate the two 1 liter collapsible bottles with air prior to adding liquids<br />

3) Put on safety goggles and gloves.<br />

4) Fill out initial information in field notes.<br />

5) Flush port: to clear line of standing water have ten times the sample volume discharged into a<br />

bucket or container prior to sampling.<br />

Sample:<br />

1) Have ship’s crew fill all and cap all provided bottles. Fecal bottle must not be rinsed,<br />

filled only once, and not overflow. Use filled <strong>12</strong>5ml unpreserved bottle to perform test<br />

below in the following order:<br />

2) Chlorine test: Take one of the strips and place in <strong>12</strong>5 ml unpreserved sample bottle.<br />

Move strip back and forth for 30 seconds. Remove and compare results to color chart on<br />

the bottle, find the closest color match. The first pad from the end is total chlorine, the<br />

second is free chlorine. Write results in filed notes and on chain of custody form.<br />

3) Record temperature. Insert thermometer up to the insertion line on thermometer.<br />

When temperature is stable (not changing) record temperature to nearest 0.5 degrees<br />

Celsius.<br />

4) pH test: Dip one pH test strip into <strong>12</strong>5ml unpreserved sample bottle. Read when there is<br />

no further color change, and while still moist. Compare to color chart on container.<br />

5) Tamper-resistant seal- use to seal cooler when all samples are placed inside.<br />

6) Properly dispose of test strips and gloves.<br />

Sample drop off information:<br />

Contact DEC or Crowley contact to arrange a sample-pick up from the vessel. Provide vessel<br />

location information.<br />

2<br />

241

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