Abstracts - Association for Chemoreception Sciences

#P66 POSTER SESSION II:
OLFACTORY PHYSIOLOGY & CELL BIOLOGY;
TASTE MOLECULAR GENETICS;
CHEMESTHESIS & TRIGEMINAL
Integrating heterogeneous Odor Response Data into a
common Response Model: A DoOR to the Complete
Olfactome
C Giovanni Galizia 1 , Daniel Munch 1 , Martin Strauch 1 ,
Anja Nissler 2 , Shouwen Ma 1
1
Universitat Konstanz Konstanz, Germany, 2 Humboldt
Universität Berlin, Germany
We have developed a new computational framework for merging
odor response datasets from heterogeneous studies, creating a
consensus meta-database, the Database of Odor Responses
(DoOR). As a result we obtained a functional atlas of all available
odor responses in Drosophila melanogaster. Both the program and
the dataset are freely accessible and downloadable on the Internet
(http://neuro.uni-konstanz.de/DoOR). The procedure can be
adapted to other species, thus creating a family of “olfactomes” in
the near future. D. melanogaster was chosen because of all species
in this one we are closest to the complete olfactome, with the
highest number of deorphanized receptors available. The database
guarantees long-term stability (by offering time-stamped,
downloadable versions), up-to-date accuracy (by including new
datasets as soon as they are published), and portability (for other
species). We hope that this comprehensive repository of odor
response profiles will be useful to the olfactory community and to
computational neuroscientists alike. Acknowledgements: BMBF
grant 576/07 to MS and CGG, and DFG grant GA524/7-1 to
DM. MS is an associated member of the DFG research training
group GK-1042
#P68 POSTER SESSION II:
OLFACTORY PHYSIOLOGY & CELL BIOLOGY;
TASTE MOLECULAR GENETICS;
CHEMESTHESIS & TRIGEMINAL
Splice variants of the Ca 2+ -activated Cl - channel Anoctamin 2
Samsudeen Ponissery Saidu 1 , Aaron B. Stephan 2 , Sonia M.
Caraballo 2 , Haiqing Zhao 2 , Johannes Reisert 1
1
Monell Chemical Senses Center Philadelphia, PA, USA,
2
Department of Biology, The Johns Hopkins University
Baltimore, MD, USA
Olfactory receptor neurons (ORNs) in vertebrates use a calciumactivated
chloride current to amplify the receptor potential in
response to odor stimulation. Anoctamin 2 (ANO2, also called
TMEM16B) has recently been proposed to be the long-sought
calcium-activated chloride channel in the ORN (Stephan et al,
2009). Biophysical properties of heterologously-expressed ANO2
channels are similar to those of the native calcium-activated
channel, but with one noticeable difference: the native channel
inactivates during prolonged opening at positive membrane
potentials, whereas the heterologously expressed ANO2 channel
does not. Different splice variants of ANO2 transcripts have been
found in tissues including the olfactory epithelium and the retina.
Using molecular techniques we found two alternative, hitherto
not described ANO2 transcripts in ORNs, with alternative
transcription initiation sites. Expression in a heterologous system
and excised patch recordings yielded functional channels with
biophysical properties largely similar to previously described
ANO2 splice variants. To better understand the functional
significance of various splice variants, we will further investigate
biophysical properties of splice variants. Acknowledgements: This
work is supported by a Morley Kare Fellowship, the Human
Frontiers Science Organization and NIH R01 DC007395.
P O S T E R S
#P67 POSTER SESSION II:
OLFACTORY PHYSIOLOGY & CELL BIOLOGY;
TASTE MOLECULAR GENETICS;
CHEMESTHESIS & TRIGEMINAL
The multiple PDZ domain protein 1 (MUPP1) – mediator of
the olfactosome?
Sabrina Baumgart 1 , Robert Menzler 1 , Ruth Dooley 2 , Hanns Hatt 1 ,
Eva Maria Neuhaus 3
1
1 Bochum, Germany, 2 Dublin, Ireland, 3 Berlin, Germany
The complex olfactory signal transduction pathway enables
mammals to detect and discriminate between thousands of
different odorants. But how the individual components of this
complex signaling cascade get into close proximity to each other is
an unsolved question. We recently showed that a PDZ protein
called MUPP1 (multiple PDZ domain protein 1) is an interaction
partner of olfactory receptors and thereby a putative mediator of a
so called olfactosome (Baumgart et al, FEBS journal, Dec 2009).
This scaffolding protein consists of 13 single PDZ domains and is
known to be a mediator of diverse GPCR based signalling
networks. We demonstrated that this scaffolding protein is highly
expressed in the dendritic knobs and cilia of olfactory sensory
neurons of mice. Further, we could show that different ORs are
able to interact with MUPP1. In a new peptide microarray
approach we investigate putative interactions of a great variety of
ORs of all different known subfamilies in the mouse genome. In
addition, we investigate other signaling components for their
putative presence in the MUPP1 based complex by protein
microarrays, as well as by direct protein-protein interaction
assays.
Abstracts are printed as submitted by the author(s)
#P69 POSTER SESSION II:
OLFACTORY PHYSIOLOGY & CELL BIOLOGY;
TASTE MOLECULAR GENETICS;
CHEMESTHESIS & TRIGEMINAL
An electroolfactogram (EOG) study of odor response maps
from the mouse olfactory mucosa?
David M Coppola 1 , Sarah M Held 1 , David A Brooks 1 ,
Chris T Waggener 2
1
Department of Biology, Randolph Macon College Ashland, VA,
USA, 2 Department of Biology, Virginia Commonwealth
University Richmond, VA, USA
Olfactory sensory neuron (OSN) responses measured at the
population level tend to be spatially heterogeneous in vertebrates
and response “maps” vary with odor. One proximate explanation
for this heterogeneity comes from evidence that olfactory receptor
genes in rodents are expressed in OSN populations that are
spatially restricted to one of four zones in the nasal cavity. An
ultimate explanation for response anisotropy posits that it is the
signature of a supplementary mechanism for quality coding, based
on the sorptive properties of odor molecules. These theories are
difficult to assess because most mapping studies have utilized few
odors, have provided little replication, or have involved but a
single species (rat). In fact, to our knowledge, a detailed olfactory
response map has not been reported for mouse, the species in
which most of the gene localization work has been done. Here we
report the results of a study of the mouse olfactory mucosal
response map using the EOG. We focused on the medial aspect of
olfactory turbinates as viewed in midsagittal section. This limited
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