Abstracts - Association for Chemoreception Sciences

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P O S T E R S #P188 POSTER SESSION IV: CHEMOSENSORY TRANSDUCTION AND SIGNALING The Taste of Salicin in Hamsters Nicole H Strobel, Marion E Frank, Thomas P Hettinger, Bradley K Formaker Center for Chemosensory Sciences, Oral Health & Diagnostic Sciences, Dental Medicine, University of Connecticut Health Center Farmington, CT, USA Salicin is a b-glucopyranoside derived from willow tree bark. The taste of salicin, bitter to humans, is thought to be mediated by the TAS2R16 receptor, which has orthologs in rats and mice with ~50% amino acid identity. In order to examine the behavioral taste of salicin in the golden Syrian hamster (Mesocricetus auratus) we conditioned 7 animals to 10 mM salicin (experimental group) and 7 animals to deionized water (control group). After a single conditioning trial, all animals were tested twice with the following test stimuli (TS): 10 mM salicin, 2 analogs of salicin: 10 mM phenyl-b-D-glucopyranoside (P-b-D) and 10 mM phenyl-a-Dglucopyranoside (P-a-D), 0.3 mM quinine•HCl (QHCl), 3 mM caffeine, 1 mM sucrose octaacetate, 1 mM saccharin, 100 mM sucrose, 100 mM NaCl and deionized water. P-b-D tastes bitter to humans, but P-a-D does not. The TS were presented in a counterbalanced order. Because the limited amount of P-a-D available was insufficient to complete 2 test trials in all animals, it was omitted from the ANOVA. The aversion learned to salicin (60% suppression, p
#P191 POSTER SESSION IV: CHEMOSENSORY TRANSDUCTION AND SIGNALING Both Warming and Cooling Enhance the Bite of Carbonation Paul M Wise, Bruce Bryant Monell Chemical Senses Center Philadelphia, PA, USA Carbon dioxide, which gives carbonated beverages their distinct bite, probably stimulates through transient acidification of the mucosa. Work by Barry Green and colleagues showed that cooling carbonated water to below tongue temperature enhanced carbonation bite, suggesting that cool-sensitive fibers play a role. Other work suggests that TRPV1-expressing fibers, which are sensitized by warming, account for some of the response to carbon dioxide. Accordingly, we tested the hypothesis that warming carbonated water above tongue temperature would also enhance carbonation bite. Carbonated water was presented at four nominal concentrations (0.0, 2.0, 2.8, and 4.0 v/v) X five temperatures (18.3, 33.9, 38.95, 44.9, and 48.2 ºC). Temperature spanned a range from well below to well above tongue temperature. Subjects dipped the anterior portion of their tongues into cups of carbonated water, and rated carbonation bite. Each subject received all combinations of temperature and carbonation level (within-subjects design). Rated carbonation bite followed a U-shaped (significant quadratic) trend with respect to temperature. Thus, we replicated the finding that cooling enhances carbonation bite, and extended previous results to show that warming also enhances bite. Measured carbonation levels decreased somewhat as temperature increased, perhaps from more rapid loss after opening sample bottles. However, the degree of CO2 loss was relatively small, and would be expected to undermine carbonation bite at higher temperatures rather than enhance it. Accordingly, we conclude that fibers sensitive to both cooling and warming (or both cool- and warm-sensitive fibers) play a role in carbonation sensation. Acknowledgements: Supported in part by The Coca-Cola Company #P192 POSTER SESSION IV: CHEMOSENSORY TRANSDUCTION AND SIGNALING Expression, Solubilization, Purification and Reconstitution of the Human Epithelial Sodium Channel Involved with Salty Taste Jesusa S. Josue 1 , Yuri Kaulin 2 , Joseph G. Brand 1 1 Monell Chemical Senses Center Philadelphia, PA, USA, 2 Thomas Jefferson University Philadelphia, PA, USA heterotrimer channel, the ENaC-containing liposomes were fused with a lipid bilayer. Recombinant a, b, g displayed single-channel Na conductance of 21 pS in 200 mM NaCl, and was blocked by low concentrations of amiloride (apparent Ki amiloride, 0.5 uM). Reconstituted d,b,g-ENaC protein resulted in smaller single channel conductance of 17 pS and higher Ki amiloride of 5 uM, consistent with expected activity of d versus a. These observations were similar to those observed from reconstitution of membrane vesicles prepared directly from d-b-g or a-b-g transfected Sf9 cells. Successful reconstitution of purified subunits means we can study the channel in isolation, modifying its environment with impunity. #P193 POSTER SESSION IV: CHEMOSENSORY TRANSDUCTION AND SIGNALING Taste-evoked chorda tympani responses to CaCl 2 are larger in PWD/PhJ than in C57BL/6J mice Chandra M Cherukuri 1 , Stuart A McCaughey 2 , Michael G Tordoff 3 1 Dept. of Physiology and Health Science, Ball State University Muncie, IN, USA, 2 Center for Medical Education, IUSM-Muncie at Ball State University Muncie, IN, USA, 3 Monell Chemical Senses Center Philadelphia, PA, USA PWD/PhJ (PWD) mice show preferences for calcium-containing compounds in two-bottle tests with water, whereas C57BL/6J (B6) mice avoid them. It is not known whether this behavioral difference depends on taste sensation, although taste plays an important role in guiding calcium intake in general. We measured taste-evoked chorda tympani (CT) responses in male B6 and PWD mice in order to examine whether peripheral gustatory events differ between the strains. Each mouse was anesthetized and its right chorda tympani nerve was accessed through the ear in order to record whole-nerve responses as solutions were flowed over the tongue. Responses were significantly higher in PWD than B6 mice for CaCl2, MgCl2, citric acid and quinine, but did not differ between the strains for sucrose and NaCl. These findings were consistent with results from two-bottle choice tests; relative to the B6 strain, the PWD strain had higher preferences for CaCl2, MgCl2, citric acid and quinine, but not for sucrose or NaCl. These data suggest that differences in peripheral events, such as taste transduction, contribute to the differences between B6 and PWD mice in preferences for taste solutions. Acknowledgements: Funding provided by NIH RO1 DK-46791 P O S T E R S Human fungiform taste bud cells express the heterotrimeric epithelial sodium channel (ENaC), of subunits delta, beta and gamma (d,b,g). Single taste cell PCR rarely showed complete alpha (a) transcript. In rodents, the d gene is an unexpressed pseudogene and the tongue ENaC is a heterotrimer of a b g. The Na response in rodents is sensitive to amiloride while that of human is (generally) not. In other body tissues, the ENaC containing delta is known to be less sensitive to amiloride than that containing alpha, but is, nevertheless, still demonstrably sensitive to amiloride. To investigate this disparity, we expressed and purified the a, d, b and g units through Sf9 cells and reconstituted these into a lipid bilayer. We co-expressed Sf9 insect cells with three baculoviruses, each encoding individual human ENaC subunits, a, b, g, and d, b, g. The cells were lysed into detergent buffer, the subunits purified by affinity chromatography, and the detergent removed by dialysis out of a lipid-containing cassette. For characterization of each Abstracts are printed as submitted by the author(s) Abstracts | 91
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AChemS Association for Chemorecepti
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AChemS Association for Chemorecepti
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We are pleased to announce that sev
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#1 GIVAUDAN LECTURE Normal and Canc
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and against delta ENaC is being pur
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#13 SYMPOSIUM - GENETICS OF HUMAN O
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#19 SYMPOSIUM - CILIA, SENSORY DYSF
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#26 PLATFORM PRESENTATIONS - POLAK
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esponse selectivity for both OSNs a
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elicit glucagon-like peptide-1 (GLP
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contrast, changing the concentratio
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of OBPs, we have systematically sup
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whereas mouse and joystick reaction
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POSTER PRESENTATIONS #P1 POSTER SES
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PROP strips and PROP solutions. PAV
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#P11 POSTER SESSION I: TASTE IMAGIN
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#P17 POSTER SESSION I: TASTE IMAGIN
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TGI. The present study investigated
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delivered in the scanner intra-oral
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Page 45 and 46: #P48 POSTER SESSION I: TASTE IMAGIN
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Page 53 and 54: hours later, the levels of prolifer
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Page 59 and 60: cells within gustatory papillae, we
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Page 63 and 64: gene, HMBS was used. All primers we
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Page 85 and 86: #P166 POSTER SESSION IV: CHEMOSENSO
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Page 99 and 100: #P210 POSTER SESSION V: CENTRAL OLF
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Page 121 and 122: #P278 POSTER SESSION VI: PERIPHERAL
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differentiated patients with AD (M=
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pleasantness 3)of mixtures (A,B), A
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#P356 POSTER SESSION VII: OLFACTORY
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#P362 POSTER SESSION VII: OLFACTORY
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Index Aarts, H - P328 Abe, K - P88,
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Haase, L - P3, P4, P29, P355 Haddad
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Murata, Y - P272 Murphy, C - P3, P4
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Veldhuizen, M - P7, P24, P25, P242,
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Registration 7:30 am to 1:00 pm, 6:
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See you next year! AChemS 33rd Annu
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