THE CONFERENCE PROCEEDINGS - IFEAT

So these examples will give you an idea of what sort of compounds and what sort of limitations ofthese so-called geography and supply chain are limiting its use to commoditise, and those are thethings that my company is trying to use.So this is a quickoversimplification of the mostcomplex thing we are doing.Because we started in 2004, andwe are still learning everysingle day, so what do we do?This is nothing but acombinatorial genomics that weassembled in baker’s yeast, sowe can identify multiplesources of genes from differenttypes of species, all natural andliving. We can stitch themtogether, put them in yeast,make the desired compound byidentifying what we have to do,and then we can de-replicate(#$$01-&;0,02&&>&2?,-@02+A0&%.BC1+%$&&1@.#)#2#)02&D.#)&1#)*+,%B#,2&#D&;0,02&!"#$"%&'(#)(*+,%(-&.%,+(*%$&!"#$"%&'&(#)*+,%-#.+%$&/0,#)+12&+,&3%40.52&60%2-&(.0%-0&*+$$+#,2&#D&10$$2&&E+-@&,0E&;0,0&0,1#F0F&=%-@E%?2&G&&%,F&20$01-&-@0&*02-&the process to find out how we have done it. And by this we can make one particular yeast, make theproduct that we desire, which ultimately can be scaled up. Which means you use beer, I mean everysingle day a brewery is going to brew tonnes and tonnes of beer there, yeast is the organism, so whenyou want to make a compound, this is going to be very similar to the way beer is brewed, but insteadof making alcohol there you will be making one of the compounds which is required. It’s a very highlyIP focused area, and we as a company also spend a lot of time trying to navigate through the maze ofpatents which are there before you can actually produce something which will be useful for theindustry.The next two slides explain how we do it. I have used the example of Rebaudioside This is a problemthat was not solved for more than two decades, so for twenty years people have been trying to figureout whether they can clone the genes that are required to make the compound Rebaudioside. We as acompany did this in about 18 months using all the resources and the manpower that we have in ourhands.The reason we did it is because we knew that it is the most important sweetener molecule in the Steviafamily. We felt that they will have to be individually made, because when you put them all together itends up giving you a bit of an aftertaste which a lot of the beverage companies in the world are notexactly excited about, because their customers do tend to have something in their mouths after theydrink their most preferred drink. So one of the ways you have to solve that is to probably separate outthe different compounds, and its not easy to do it otherwise, because you have such low quantities, sothe best thing to do is to probably synthesize them, put them in yeast and make them ferment.The three bullet points summarise what this technology can do. The first one is that you can buildproduction routes that are not in existence any more. In the sense that if there is an enzyme which hasto work faster, it has to have a better conversion, a better industrial proficiency, then you end up doingthis through this science.7)%$$&8#$019$02&:%.;0-&&&21%$0'9=&Slide 7206