Guidelines for care & Use of Dry Solvent Stills [Example]

1. Dr. Alexander – Lab Room #108B
Lab Personnel Phone Numbers
Laboratory Room Number 108 Salem Hall, Reynolda Campus
Laboratory phone #(or nearest building phone #) 758-5576
Laboratory Instructor Dr. Rebecca Alexander Office Phone 758-5568
Office Room # 108A Home Phone 723-3532
Students Assigned to this Laboratory, Name Home Phone Number
including Graduate Students,
Undergraduates and Post Doctorates
Michael E. Budiman 703-0283
Susan A. Walker 704-639-0927
Lela Lackey
Pam Edwards
Brief Description of Research Conducted in This Lab
INCLUDING POSSIBLE PROBLEMS FOR FIREFIGHTERS WITHIN:
Research includes mechanistic investigation of enzymes involved in the protein
biosynthesis pathway. Operations include growth of bacterial strains (non-pathogenic E.
coli) for purification of proteins and nucleic acids. Radioactive materials are used in
minute quantities for trace labeling and kinetic assays. Our license covers use of 32 P,
35 S, and 3 H radionuclides. 32 P is a high energy beta emitter whose decay particles are
stopped by 0.25 inches of plexiglass. 35 S and 3 H are low energy beta emitters whose
decay particles are stopped by paper or clothing. Our stock vials (containing less than 1
mL each) are stored in locked, shielded containers in the lab refridgerator and freezer.
Small quantities of flammable solvents (methanol, ethanol, acetone, acetonitrile) are
stored in the flammable solvents cabinet (under the hood) or in the refridgerator (rated for
flammables).
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