Guidelines for care & Use of Dry Solvent Stills [Example]

2. Dr. Bierbach – Lab Room #107
SPECIFIC PROCEDURES FOR SAFE REMOVAL OF HIGHLY TOXIC WASTE
Consult with your research advisor concerning proper methods for packaging and storing extremely
hazardous waste. Research usually involves working with selective types or classes of chemicals. Prepare
brief summaries in this section for handling spent chemicals peculiar to your lab.
Two categories of hazardous waste are generated in room 107. The first is radioactive waste, which will
be disposed of according to the requirements of the office of radiation safety as outlined in the user’s
license. Briefly, short half-life ( 32 P ) will be stored in a shielded container. This will be collected at
regular intervals by employees of the office of radiation safety.
The second category is ethidium bromide used to detect nucleic acids by gel electrophoresis. The
quantities used (except for stock quantities) are on the order of 0.05% in water or gel. Low concentration
liquid waste will be decontaminated by household bleach and disposed of in the drain after
decontamination.
Beirbach‟s chemical waste, taken to the waste storage area in room # 20
Dr. Beirbach’s chemical waste
HAZARDOUS WASTE, DATE_________
� Dichloromethane
� Chloroform
� 9-Aminoacridine (1 – 3%)
� Dimethylformamide (1 – 5%)
HAZARDOUS WASTE, DATE_________
� 1,4-Dioxane
� Tetrahydrofuran
� Diethyl ether
� 9-Aminoacridine (1 – 3%)
� Dimethylformamide (1 – 5%)
HAZARDOUS WASTE, DATE__________
� Ethanol
� Acetone
� Methanol
� 9-Aminoacridine (1 – 3%)
� Dimethylformamide (1 – 5%)
HAZARDOUS WASTE, DATE__________
Water with 1 to 5% of each of:
� Cobalt chloride
� Chromium chloride
� Sodium sulfate
� Calcium chloride
� Magnesium sulfate
� Sodium chloride
� 9-Aminoacridine
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