Guidelines for care & Use of Dry Solvent Stills [Example]

5. Dr. Colyer – Lab Room # 114
SPECIFIC PROCEDURES FOR SAFE REMOVAL OF HIGHLY TOXIC WASTE
Consult with your research advisor concerning proper methods for packaging and storing extremely
hazardous waste. Research usually involves working with selective types or classes of chemicals. Prepare
brief summaries in this section for handling spent chemicals peculiar to your lab.
The Colyer lab (Salem 114) binds organic dyes to proteins and then separates them using capillary
electrophoresis. The organic dyes are typically prepared in methanol, DMSO, or DMF and later diluted in
water, or aqueous buffers. Extremely small amounts of waste are produced at any given time, due to the
total of 3-10 mL of buffer used by a researcher on any given day. Due to the basic fuctioning of the system,
small amounts of protein will be disposed of with the buffer waste. The final concentrations of protein tend
to be in the pico-femtomolar concentration in the buffer or organic hazardous waste systems. The pure
proteins are prepared in water, stored in the refrigerator or freezer, and disposed of by neutralization in
50% bleach solution.
Typical chemical waste generated in this lab is as follows. It will be labeled as Hazardous Waste, the
contents of each properly bottle sealed, and all will be taken downstairs to the solvent room # 20 to await
removal by a chemical waste company:
HAZARDOUS WASTE
1) Methanol and Ethanol with organic dyes, as follows:
� DIMETHYL-SULFOXIDE (DMSO), less than 1%
� N,N-DIMETHYLFORMAMIDE (DMF), less than 1%
� SQUARYLIUM RED-1C, ORGANIC DYE, less than 1%
� SQUARYLIUM RED-3, ORGANIC DYE, less than 1%
� NN-127, ORGANIC DYE, less than 1%
� CALCAFLUOR, ORGANIC DYE, less than 1%
� FQ, ORGANIC DYE, less than 1%
� FLUORESCAMINE, ORGANIC DYE, less than 1%
HAZARDOUS WASTE
2) Aqueous with organic dyes, as follows:
� METHANOL, less than 1%
� DIMETHYL-SULFOXIDE (DMSO), less than 1%
� N,N-DIMETHYLFORMAMIDE (DMF), less than 1%
� SQUARYLIUM RED-1C, ORGANIC DYE, less than 1%
� SQUARYLIUM RED-3, ORGANIC DYE, less than 1%
� NN-127, ORGANIC DYE, less than 1%
� CALCAFLUOR, ORGANIC DYE, less than 1%
� FQ, ORGANIC DYE, less than 1%
� FLUORESCAMINE, ORGANIC DYE, less than 1%
HAZARDOUS WASTE
3) Methanol and Ethanol with organic dyes and proteins, as follows:
� DIMETHYL-SULFOXIDE (DMSO), less than 1%
� N,N-DIMETHYLFORMAMIDE (DMF), less than 1%
� SQUARYLIUM RED-1C, ORGANIC DYE, less than 1%
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