Landfills and waste water treatment plants as sources of ... - GKSS

STUDY 1: LANDFILLS AS SOURCES
(Supelco, Munich, Germany; 30 m x 0.25 mm x 0.25 μm). Samples were also measured in
NCI to confirm FASAs. Details on instrumental conditions were reported by Dreyer et al.
(2008).
PFC air samples from the particle phase were analysed using high performance liquid
chromatography (HPLC, HP100, Agilent, Waldbronn, Germany) coupled to an triplequadrupole
tandem mass spectrometer (MS/MS, Applied Biosystems, MDS SCIEX,
Darmstadt, Germany) using negative electrospray ionisation and the multiple reaction
monitoring (MRM) mode. Separation was done on a Phenomenex Synergi Hydro RP 80A
column (Phenomenex, Aschaffenburg, Germany) with a length of 150 mm and a inner
diameter of 2 mm connected to a guard column Phenomenex Synergi 2μ Hydro RO Mercury
(200 mm lengths and 2 mm inner diameter). Detailed instrumental conditions can be found in
Ahrens et al. (2007).
Measurement of PBDEs and musk fragrances from gas- and particle-phase samples was
performed separately using GC-MS in the EI mode (musk fragrances) and NCI mode
(PBDEs) using the previously optimized instrumental method described in chapter 3.
4.2.7 Quantification
Quantification was based on peak areas. Peak integration was done using MSD Chemstation
(Agilent Technologies, version D.02.00.275) for all samples measured by GC-MS. HPLC
samples were integrated and quantitated with Analyst (Applied Biosystems, MSD SCIEX,
version 1.4.1). Mass fragments of PBDEs and musk fragrances used for quantification are
listed in section 3.3.1 (musk fragrances, PBDEs) and in the supporting information (PFCs).
Analyte concentrations were calculated using the internal standard method and a seven point
calibration. Target compounds were quantified with an S/N>10 and detected with S/N>3. The
PFC method quantification limits (MQL) were