Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
• Step 5Ad<strong>di</strong>tion of dNTPs is performed sequentially. It should be noted thatdeoxyadenosine -thio triphosphate (dATP·S) is used as a substitutefor the natural deoxyadenosine triphosphate (dATP) since it isefficiently used by the DNA polymerase, but not recognized by theluciferase. As the process continues, the complementary DNA stran<strong>di</strong>s built up and the nucleotide sequence is determined from the signalpeaks in the Pyrogram trace.