Appunti di Principi-BiolMol CTF - Capitolo3 - Omero

ABI SOLiD (Seq by Oligo Ligation/Detection)• Clonal bead library via emulsion PCR.• The actual base detection is no longer done by the polymerasedrivenincorporation of labeled dideoxy terminators.• SOLiD uses a mixture of labeled oligonucleotides and queriesthe input strand with ligase.• Each base is interrogated twice:– built-in error checking capability that distinguishes betweenmeasurement errors and true polymorphisms;– detection of more complicated variations.