Appunti di Principi-BiolMol CTF - Capitolo3 - Omero

Sequencing by LigationPrimers hybridize to the P1 adapter sequence on the templatedbeads .A set of four fluorescently labeled di-base probes compete forligation to the sequencing primer. Specificity of the di-base probeis achieved by interrogating every 1st and 2nd base in eachligation reaction.Multiple cycles of ligation, detection and cleavage are performedwith the number of cycles determining the eventual read length.Following a series of ligation cycles, the extension product isremoved and the template is reset with a primer complementaryto the n-1 position for a second round of ligation cycles.