Appunti di Principi-BiolMol CTF - Capitolo3 - Omero

More documents

Recommendations

Info

Illumina Genome Analyzer 1 “flow cell” = 8 “lanes” 1 lane = ~10-30 million “reads” ~5-20 million “mapped reads” 36bp, 50bp, 75bp, 100bp Single-end (SE) or Paired-ends (PE) 1 lane: $800-$2000 Multiplexing
Illumina:Sequencing-bysynthesis
Page 3 and 4:
Impieghi della PCR• Strategie di
Page 5 and 6:
TemperaturePCR100Melting94 o C500T
Page 7 and 8:
TemperaturePCR10050Melting94 o CAnn
Page 9 and 10:
TemperaturePCR10050Melting94 o CAnn
Page 11 and 12:
Dopo n cicli il miscuglio di reazio
Page 14 and 15:
Fasi della PCR Denaturazione (melti
Page 16 and 17:
• * Si possono usare al posto del
Page 18 and 19:
Nested PCR
Page 20:
Hot Start PCR
Page 23:
Sintesi del cDNA
Page 27:
• L’elettroforesi su gel dei pr
Page 30 and 31:
IMPIEGHI DELLA PCRDiagnosi infezion
Page 33 and 34:
Test geneticoAmplificazionemediante
Page 35 and 36:
PCR-ARMS (Amplification RefractoryM
Page 37 and 38:
PCR-ARMS (Amplification RefractoryM
Page 39 and 40:
PCR-SSCP (Single Strand Conformatio
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
PCR-HAD (HeteroDuplex Analysis)•
Page 47 and 48:
PCR-HAD (HeteroDuplex Analysis)
Page 49 and 50:
PCR-DGGE (Denaturation Gradient Gel
Page 51 and 52:
PCR-DGGE (Denaturation Gradient Gel
Page 53 and 54:
Resa della PCRResa effettiva: effet
Page 55 and 56:
Soluzioni per PCR quantitativa Util
Page 57 and 58:
Perché Real-Time?• Misura l'ampl
Page 59 and 60:
Analisi tramite software
Page 61 and 62:
SYBR greenUtilizzauna molecola fluo
Page 63 and 64:
SYBR green Dopo l’annealing dei p
Page 65 and 66:
SYBR green Metodica semplicePossono
Page 67:
Sonde TaqMan La sonda di tipo TaqMa
Page 71 and 72:
ForwardProbeReverse
Page 73 and 74:
Reporter-QuencherDyeQuencher5,6 FAM
Page 75 and 76: Real-Time PCR:attività 5’>3’ e
Page 77 and 78: Real-time PCR: reagenti COMPONENTI
Page 79 and 80: Curve di amplificazioneFluorescenza
Page 81 and 82: QuantificazioneASSOLUTA i campioni
Page 83 and 84: Quantificazione relativaPlot di amp
Page 85 and 86: Sonde FRET(FluorescenceResonanceEne
Page 87 and 88: Molecular Beacons Durante lo step d
Page 89 and 90: Probes : ScorpionsStep 1Lo Scorpion
Page 91 and 92: Step 3Probes : ScorpionsRaffreddand
Page 93 and 94: Real-Time PCR: applicazioni‣ Quan
Page 95 and 96: Analisi della sequenzanucleotidica
Page 97 and 98: ddNTP
Page 99 and 100: • Si suddivide quindi la miscela
Page 101 and 102: ddCTPddCTPddCTPPRIMERDNA Polimerasi
Page 103 and 104: • Le diverse catene polinucleotid
Page 105 and 106: Metodo di sequenziamentodel DNA “
Page 107 and 108: Sequenziamento automatizzato con ma
Page 109 and 110: Le emissioni fluorescenti vengono c
Page 111 and 112: Metodo di Sequenziamento di Maxame
Page 113 and 114: Un altro metodo per la determinazio
Page 115 and 116: • Step 1A sequencing primer is hy
Page 117 and 118: • Step 3ATP sulfurylase converts
Page 119: • Step 5Addition of dNTPs is perf
Page 123 and 124: What is Next-Generation Sequencing?
Page 125: Illumina sequencing technology• L
Page 129 and 130: Illumina sequencing technology• S
Page 131 and 132: Overview of ABI SOLiD SequencingChe
Page 133 and 134: Bead DepositionDeposit 3’ modifie
Page 136 and 137: SOLiD TechnologyLigation-basedchemi
Page 138 and 139: Exact Call ChemistryUp to 99.99% ac
Page 140 and 141: Latest Platforms:Illumina HiSeq:•
Page 142: Campi di applicazione delle piattaf
show all

Appunti di Principi-BiolMol CTF - Capitolo3 - Omero

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?