- Page 3 and 4: Impieghi della PCR• Strategie di
- Page 5 and 6: TemperaturePCR100Melting94 o C500T
- Page 7 and 8: TemperaturePCR10050Melting94 o CAnn
- Page 9 and 10: TemperaturePCR10050Melting94 o CAnn
- Page 11 and 12: Dopo n cicli il miscuglio di reazio
- Page 14 and 15: Fasi della PCR Denaturazione (melti
- Page 16 and 17: • * Si possono usare al posto del
- Page 18 and 19: Nested PCR
- Page 20: Hot Start PCR
- Page 23: Sintesi del cDNA
- Page 27: • L’elettroforesi su gel dei pr
- Page 31: PCR-RFLPThe polymorphism results fr
- Page 34 and 35: PCR-ARMS (Amplification RefractoryM
- Page 36 and 37: PCR-ARMS (Amplification RefractoryM
- Page 38 and 39: PCR-ARMS (Amplification RefractoryM
- Page 40 and 41: PCR-SSCP (Single Strand Conformatio
- Page 42 and 43: PCR-SSCP (Single Strand Conformatio
- Page 44 and 45: PCR-SSCP (Single Strand Conformatio
- Page 46 and 47: PCR-HAD (HeteroDuplex Analysis)•
- Page 48 and 49: PCR-HAD (HeteroDuplex Analysis)
- Page 50 and 51: PCR-DGGE (Denaturation Gradient Gel
- Page 52 and 53: Resa della PCRResa teorica: 2 n P =
- Page 54 and 55: PCR productIl plateau non dipende d
- Page 56 and 57: RT-PCR convenzionaleReversetrascrip
- Page 58 and 59: RT-PCR quantitativa• Rilevamento
- Page 60 and 61: Chimiche fluorescenti per PCR Real-
- Page 62 and 63: SYBR green All’inizio del process
- Page 64 and 65: SYBR green Durante l’elongazione
- Page 66 and 67: La Real-Time PCR si può realizzare
- Page 69: Dimensioni dell’amplicone
- Page 72 and 73: Sonda TaqMan Presenta all’estremi
- Page 74 and 75: Reporter-Quencher5’ REPORTER (R):
- Page 76 and 77: L’aumento di fluorescenza del Rep
- Page 78 and 79:
Run Real-Time thermal cycle3 min95
- Page 80 and 81:
Plot di amplificazioneLinea soglia
- Page 82 and 83:
Ct35Quantitativa assoluta10 110 225
- Page 84 and 85:
Quantificazione relativa: analisi d
- Page 86 and 87:
Molecular Beacons I "molecular beac
- Page 88 and 89:
Laprogettazionedellescorpion probes
- Page 90 and 91:
Step 2Probes : ScorpionsDurante il
- Page 92 and 93:
Riassumendo:Metodi di rilevamento d
- Page 94 and 95:
MRD (MinimalResidualDisease): Quota
- Page 96 and 97:
• Uno dei metodi più comunemente
- Page 98 and 99:
Il principio del procedimento può
- Page 100 and 101:
Terminazione della catena
- Page 102 and 103:
Schema di sequenziamento a terminaz
- Page 104 and 105:
Metodo di sequenziamentodel DNA “
- Page 106 and 107:
• Alla rilevazione per autoradiog
- Page 108 and 109:
Detection of FluorescentlyTagged DN
- Page 110 and 111:
Fluorescent DNA Sequencing Data
- Page 112 and 113:
Metodo diSequenziamentochimico seco
- Page 114 and 115:
Le tappe del pyrosequencing possono
- Page 116 and 117:
• Step 2The first deoxribonucleot
- Page 118 and 119:
• Step 4Apyrase, a nucleotide-deg
- Page 122 and 123:
• Il pyrosequencing ha notevoli v
- Page 124 and 125:
Illumina was founded in April 1998
- Page 126 and 127:
Illumina Genome Analyzer 1 “flow
- Page 128 and 129:
Illumina:Sequencing-bysynthesis
- Page 130 and 131:
ABI SOLiD (Seq by Oligo Ligation/De
- Page 132 and 133:
Emulsion PCR/Bead EnrichmentPrepare
- Page 134:
Sequencing by LigationPrimers hybri
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Primer ResetFive rounds of primer r
- Page 139 and 140:
Working in “Colorspace”
- Page 141 and 142:
Rapid Decrease in Cost•The Human