Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
Appunti di Principi-BiolMol CTF - Capitolo3 - Omero
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SOLiD TechnologyLigation-basedchemistry with <strong>di</strong>baselabelled probesOligos:– Positions 1-2 (from 3’ side): one of 16 <strong>di</strong>nucleotides– Positions 3-5: degenerate (Ns)– Positions 6-5’: degenerate and holds one of four fluorescent dyes• 5-7 ligation reactions are followed by a reset cycle• Next a new initial primer is used that is N-1 in length
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