Appunti di Principi-BiolMol CTF - Capitolo3 - Omero

Primer ResetFive rounds of primer reset are completed for each sequencetag . Through the primer reset process, virtually every base isinterrogated in two independent ligation reactions by twodifferent primers.For example, the base at read position 5 is assayed by primernumber 2 in ligation cycle 2 and by primer number 3 inligation cycle 1. This dual interrogation is fundamental to theunmatched accuracy characterized by the SOLiD System.