ÖGMKG - und Gesichtschirurgie

Kolk, Andreas
Der modifizierte nicht-virale Gentransfer von BMP-2 aus einer biodegradierbar beschichteten
Titanoberfläche konnte erstmals erfolgreich in vitro und in vivo etabliert werden. COPGROG´s
wie auch PDLLA-Oberflächen schützen den Vektor im Vergleich zu konventionellen Gen-
vektoren vor Protein bedingter Destabilisierung. Tierexperimentell konnte ein gegenüber der
Kontrollgruppe signifikant gesteigerter osteoinduktiver Effekt ohne überschießendes Wachstum
nachgewiesen werden, während das rekombinante Protein zu einer schnelleren, aber auch
überschießenden, unkontrollierten Neubildung führte.
Pautke, Christoph
Polychrome sequential labelling of bone using different tetracyclines and
spectral image acquisition (Se07-05)
Christoph Pautke 1, Thomas Tischer 2, Stephan Vogt 2, Cornelia Haczek 1, Stefan Milz 3 An-
dreas Neff 1, Hans-Henning Horch 1, Andreas Kolk 1 (christoph.pautke@gmx.net)
1: Department of Oral and Maxillofacial Surgery, Technical University of Munich, Germany 2:
Department of Orthopedic Surgery, Technical University of Munich, Germany 3: AO Research
Institute, Davos
Background
Polychrome sequential labelling of bone is the standard investigation technique to analyse
bone growth or regeneration in vivo. Due to the fact, however, that the fluorochromes are not
approved for a human application, this technique can only be applied in animal models. An
exception are tetracycline and its derivates revealing a yellowish fluorescence. Because the
fluorescence of all tetracyclines is very similar, they cannot be distinguished by conventional
methods (different fluorochrome filter sets).
The aim of this study was therefore to establish a polychrome sequential labelling of bone using
different tetracyclines and spectral image analysis.
Material and Methods
For this purpose 15 male Wistar rats (5 weeks old, appr. 200g) received 9 different tetracyclines
(Chlortetracyclin, Chlorotetracyclin Demeclocyclin, Doxycyclin, Methacyclin, Minocyclin, Oxyte-
tracyclin, Rolitetracyclin, Tetracyclin) subcutaneously either as a single application (in order to
define the pure respective fluorochrome spectrum) or sequentially in a three day interval. Bone
specimen were harvested and embedded in methylmetacrylate. Bone Analysis was performed
using spectral image acquisition (Spectral Cube SD-300, Applied Spectral Imaging, Israel).
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Results
Each tetracycline derivate revealed a characteristic fluorescence spectrum. 4 out of the 9 tetra-
cyclines were distinguishable using spectral image analysis. After linear unmixing, the 4 suita-
ble tetracyclines could be singly depicted when applied in a polychrome sequential labelling.
Discussion
The technique of polychrome sequential labelling is feasible using different tetracycline deri-
vates and spectral image analysis. Having established this polychrome bone labelling tech-
nique, the transfer to human application is possible for the first time.