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Soil sampling and analysis The aims of this Chapter were to determine and measure the ecological variables in the soil of these plants so that these variables can be compared with observations made in vitro and ex-vitro for the same species in subsequent Chapters. 3.2 MATERIALS AND METHODS Soil samples were taken from the Nieuwoudtville Wildflower Reserve (19° 8’ E, 31° 24’ S). Soil samples were divided into two groups based on locality (the two soils used by Mr. Marinus). Sample 1 was described by Mr. Marinus as dolerite soil and sample 2 as tillite soil. Soils were analyzed by KwaZulu-Natal Department of Agricultural and Environmental Affairs Soil Fertility and Analytical Services, Pietermaritzburg. A texture test with 3 fractions produced the amount of sand (0.02 - 2 mm), fine silt (0.02 - 0.002 mm) and clay (
Soil sampling and analysis The first number of the assessment has a value from 1 to 3; 1 being non-saline, 2 potentially saline and 3 saline. The second number has a value from 4 to 6; 4 being non-sodic, 5 potentially sodic and 6 sodic. A sodic soil is defined as a soil with a low soluble salt content and a high exchangeable sodium percentage (ESP), with a usual ESP > 15 (SOIL CLASSIFICATION WORKING GROUP, 1991). A code 1,4 soil is described as suitable for irrigation, a code 2,5 soil as poorly drained and not suitable for irrigation and a code 3,6 soil as not suitable for irrigation. A pH test and a water content test were done on both samples. The pH of three different replicate samples was measured. Soil was mixed 1:1 (v/v) with water and homogenized with a stirrer apparatus. After about an hour the soil samples were vacuum filtered through Whatman No.1 filter papers and their pH was measured. The water content was calculated by weighing the soil, placing the soil in a drying oven set at 110°C and then subtracting the mass after no weight loss could be observed from the initial weight. 3.3 RESULTS The colour of the two samples suggests that the drainage conditions of sample 1 is superior to that of sample 2 (DONAHUE et al., 1983) (Figure 3.1). Sample 1 also had more leaf and root material than sample 2, suggesting that it has more organic matter, and therefore a higher nutrient content, than sample 2 (DONAHUE et al., 1983). It is notable that sample 2 appears more aggregated and dense. 94
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Propagation of Romulea species Pier
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Contents 2.7 GERMINATION PHYSIOLOGY
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Contents 5.3.1 Explants from seedli
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Abstract The suitability of various
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Declarations I Pierre André Swart,
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Declarations DETAILS OF CONTRIBUTIO
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Publications from this thesis ASCOU
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List of Figures Figure 2.13: Suther
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was significantly different from th
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List of Tables Table 2.1: Names of
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List of Abbreviations 2,4-D 2,4-Dic
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Die vlakhaas spits sy oor hy het di
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As die mens dan ook so sy dankbaarh
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Introduction where a great number o
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Introduction The subgenera Romulea
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2 Literature review Leef van daad e
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Literature review Figure 2.2: Life
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Literature review Figure 2.4: Life
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Literature review The plants are sh
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Literature review flowers (DE VOS,
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Literature review often found on cl
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Literature review flowers of R. lei
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Literature review flattened upper s
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2.2.16 Romulea tabularis Literature
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Literature review extinct, R. papyr
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Literature review R. leipoldtii occ
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Literature review Figure 2.8: Calvi
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Literature review Figure 2.14: Fras
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Figure 2.20: Nieuwoudtville weather
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Literature review Figure 2.26: Grah
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Literature review Figure 2.29: Diag
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Literature review Figure 2.30: A te
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Literature review Macronutrients ca
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Page 81 and 82: Literature review the embryo (COPEL
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Page 85 and 86: Literature review (COPELAND, 1976).
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Page 103 and 104: Literature review and the addition
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Page 107 and 108: Literature review environmental str
Page 109 and 110: 2.11 IN VITRO FLOWERING Literature
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Page 113 and 114: Subfamily Ixioideae (continued) Tri
Page 115 and 116: Literature review Table 2.8: Corm i
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Page 123 and 124: 4 Germination physiology 4.1 INTROD
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Page 127 and 128: Germination physiology (-N), phosph
Page 129 and 130: Germination physiology rosea seeds
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Page 133 and 134: Germination (%) 50 40 30 20 10 0 77
Page 135 and 136: Germination (%) 100 80 60 40 20 0 c
Page 137 and 138: Germination physiology The relative
Page 139 and 140: Germination physiology seeds of R.
Page 141 and 142: 5.2 MATERIALS AND METHODS In vitro
Page 143 and 144: In vitro culture initiation and mul
Page 145 and 146: 5.2.3 Explant comparison In vitro c
Page 159 and 160: 5.4 DISCUSSION In vitro culture ini
Page 163 and 164: 6 In vitro corm formation and flowe
Page 165 and 166: In vitro corm formation and floweri
Page 167 and 168: 6.3 RESULTS 6.3.1 Corm formation In
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In vitro corm formation and floweri
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Commercialization potential of Romu
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Commercialization potential of Romu
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BASKIN, C. C., and BASKIN, J. M. (1
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Literature cited DOWLING, P. M., CL
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Literature cited HILTON-TAYLOR, C.
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Literature cited KUMAR, A., SOOD, A
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Literature cited PIERIK, R. L. M. (
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Literature cited STEINITZ, B., COHE
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