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formation; SC = shoot cluster; SCR = shoot cluster with roots; CSCI = callus with shoot cluster initials; CIS = corm-like structure (< 10 mm); CAI = callus appearing incompetent; CPE = callus with potential embryogenesis; PDE = potential direct embryogenesis; CSGR = cultures showed growth response. Potential embryogenesis refers to cultures that appeared to develop embryolike structures (Figure 5.7). Table 6.1: The effect of different temperatures and media composition on the in vitro formation and growth of Romulea minutiflora corms. Data shows the means ± the standard error. Letters indicates significant differences between treatments according to Duncan’s multiple range test. Table 6.2: Percentage corm induction for Romulea minutiflora shoots cultured on medium supplemented with growth retardants. Table 6.3: The effect of different temperatures and media composition on the in vitro formation and growth of Romulea sabulosa corms. Data shows the means ± the standard error. Letters indicate significant differences between treatments according to Duncan’s multiple range test. Table 6.4: Cultures with multiple corm formation for Romulea sabulosa. This shows the percentage of corm formation in cultures in which corm formation observed (Total cultures with corms) and the average number of corms produced in instances of multiple corm formation. List of Tables 128 142 143 144 144 xix
List of Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid 2-iP N6(2-isopentenyl)-adenine ABA Abscisic acid BA 6-Benzyl-aminopurine Butenolide 3-methyl-2H-furo[2,3-c]pyran-2-one CaCl2 Calcium chloride CaCO3 Calcium carbonate GA Gibberellic acid HCl Hydrochloric acid IAA Indole-3-acetic acid IBA Indole-3-butryric acid Kinetin 6-Furfurylaminopurine KNO3 Potassium nitrate KOH Potassium hydroxide MemT 6-(3-methoxybenzylamino)purine MemTR 6-(3-methoxybenzylamino)-9-b-D-ribofuranosylpurine MGT Mean germination time MPa Mega Pascal MS Murashige and Skoog mT 6-(3-hydroxybenzylamino)purine mTR 6-(3-hydroxybenzylamino)-9-b-D-ribofuranosylpurine NAA Naphthaleneacetic acid NADPH Nicotinamide Adenine Dinucleotide Phosphate NaOH Sodium hydroxide nm nanometer PGR Plant Growth Regulator Thidiazuron N-phenyl-N-1,2,3,-thidiazol-5-ylurea TMS Table Mountain Sandstone TTC 2,3,5-triphenyltetrazolium chloride Zeatin Trans-6-(4-hydroxyl-3-methylbut-2enyl) aminopurine µM Micromole µm Micrometer xx
Page 1 and 2: Propagation of Romulea species Pier
Page 3 and 4: Contents 2.7 GERMINATION PHYSIOLOGY
Page 5 and 6: Contents 5.3.1 Explants from seedli
Page 7 and 8: Abstract The suitability of various
Page 9 and 10: Declarations I Pierre André Swart,
Page 11 and 12: Declarations DETAILS OF CONTRIBUTIO
Page 13 and 14: Publications from this thesis ASCOU
Page 15 and 16: List of Figures Figure 2.13: Suther
Page 17 and 18: was significantly different from th
Page 19: List of Tables Table 2.1: Names of
Page 23 and 24: Die vlakhaas spits sy oor hy het di
Page 25 and 26: As die mens dan ook so sy dankbaarh
Page 27 and 28: Introduction where a great number o
Page 29 and 30: Introduction The subgenera Romulea
Page 31 and 32: 2 Literature review Leef van daad e
Page 33 and 34: Literature review Figure 2.2: Life
Page 35 and 36: Literature review Figure 2.4: Life
Page 37 and 38: Literature review The plants are sh
Page 39 and 40: Literature review flowers (DE VOS,
Page 41 and 42: Literature review often found on cl
Page 43 and 44: Literature review flowers of R. lei
Page 45 and 46: Literature review flattened upper s
Page 47 and 48: 2.2.16 Romulea tabularis Literature
Page 49 and 50: Literature review extinct, R. papyr
Page 51 and 52: Literature review R. leipoldtii occ
Page 53 and 54: Literature review Figure 2.8: Calvi
Page 55 and 56: Literature review Figure 2.14: Fras
Page 57 and 58: Figure 2.20: Nieuwoudtville weather
Page 59 and 60: Literature review Figure 2.26: Grah
Page 61 and 62: Literature review Figure 2.29: Diag
Page 63 and 64: Literature review Figure 2.30: A te
Page 65 and 66: Literature review Macronutrients ca
Page 67 and 68: Literature review The soils of the
Page 69 and 70: Literature review The embryo is the
Page 71 and 72:
Literature review Phase 2 is seen a
Page 73 and 74:
Literature review endogenous gibber
Page 75 and 76:
Literature review tolerable level (
Page 77 and 78:
Literature review these channels pe
Page 79 and 80:
Literature review 2.8.7 Seed dorman
Page 81 and 82:
Literature review the embryo (COPEL
Page 83 and 84:
Literature review germinated under
Page 85 and 86:
Literature review (COPELAND, 1976).
Page 87 and 88:
2.9 BRIEF REVIEW OF IN VITRO CULTUR
Page 89 and 90:
Literature review (DEBERGH, 1994).
Page 91 and 92:
Literature review PIERIK (1997) sta
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Literature review The distinguishin
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Literature review The essential fun
Page 97 and 98:
2.9.3.4 Abscisic acid Literature re
Page 99 and 100:
Table 2.6: Example of a matrix to e
Page 101 and 102:
Literature review Young embryos req
Page 103 and 104:
Literature review and the addition
Page 105 and 106:
Literature review organ is then pro
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Literature review environmental str
Page 109 and 110:
2.11 IN VITRO FLOWERING Literature
Page 111 and 112:
Literature review higher regenerati
Page 113 and 114:
Subfamily Ixioideae (continued) Tri
Page 115 and 116:
Literature review Table 2.8: Corm i
Page 117 and 118:
3 Investigation into the habitat of
Page 119 and 120:
Soil sampling and analysis The firs
Page 121 and 122:
Soil sampling and analysis Table 3.
Page 123 and 124:
4 Germination physiology 4.1 INTROD
Page 125 and 126:
4.2.2 Water content and imbibition
Page 127 and 128:
Germination physiology (-N), phosph
Page 129 and 130:
Germination physiology rosea seeds
Page 131 and 132:
Germination physiology Figure 4.4:
Page 133 and 134:
Germination (%) 50 40 30 20 10 0 77
Page 135 and 136:
Germination (%) 100 80 60 40 20 0 c
Page 137 and 138:
Germination physiology The relative
Page 139 and 140:
Germination physiology seeds of R.
Page 141 and 142:
5.2 MATERIALS AND METHODS In vitro
Page 143 and 144:
In vitro culture initiation and mul
Page 145 and 146:
5.2.3 Explant comparison In vitro c
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Page 153 and 154:
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Page 157 and 158:
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5.4 DISCUSSION In vitro culture ini
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6 In vitro corm formation and flowe
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In vitro corm formation and floweri
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6.3 RESULTS 6.3.1 Corm formation In
Page 169 and 170:
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Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Commercialization potential of Romu
Page 179 and 180:
Commercialization potential of Romu
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BASKIN, C. C., and BASKIN, J. M. (1
Page 183 and 184:
Literature cited DOWLING, P. M., CL
Page 185 and 186:
Literature cited HILTON-TAYLOR, C.
Page 187 and 188:
Literature cited KUMAR, A., SOOD, A
Page 189 and 190:
Literature cited PIERIK, R. L. M. (
Page 191 and 192:
Literature cited STEINITZ, B., COHE
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