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In vitro culture initiation and multiplication
observed when it was placed on media with activated charcoal. These cultures did
not multiply well, or at all, and many became necrotic.
What appeared to be indirectly formed embryogenic tissue was observed on all
media supplemented with both kinetin and 2,4-D and its induction percentage was
highest on a medium supplemented with 2.3 M kinetin and 2.3 M 2,4-D (Table
5.1). Cultures that appeared to have direct embryogenesis was observed on media
supplemented with 4.7 M kinetin and 2.3 M 2,4-D, and 23.2 M kinetin, 4.5 M
2,4-D. Most of the cultures that appeared to have initiated embryogenesis were
observed on the latter medium.
The results of experiments that tested the effect of media supplemented with 5.4 M
NAA and 2.3 M kinetin and media with two different concentrations of BA is not
included. This is because of the vitrification observed in cultures placed on this media
and the lack of subsequent explant response by these cultures when placed on
media with no plant growth regulators.
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