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Germination (%)
50
40
30
20
10
0
77
Control (water)
68
Control (HS 50%)
Treatment
4.3.4. Ex vitro germination experiments
62
-N
64
-P
87
-K
GA3 (10 -5 M)
65
Kinetin (10 -5 M)
KNO 3 (10 -5 M)
* *
IBA (10 -5 M)
NAA (10 -5 M)
IAA (10 -5 M)
Smoke water (1:500)
Germination physiology
Butenolide (10 -8 M)
Figure 4.5: Effect of nutrients without N, P or K, plant growth promoting substances and
smoke constituents on seed germination of Romulea rosea under 16 h photoperiod at 20
± 0.5°C. A number above the standard error bar represents mean germination time and an
asterisk denotes that the treatment was significantly different from the control (water)
according to LSD test at the 5% level.
Germination was observed for the four tested Romulea species. R. diversiformis
seeds showed the highest percentage germination when placed at 10°C under
alternating light (16 h photoperiod), in the constant dark conditions and with seed
scarification treatment (Table 4.2). These results were significantly different from
other treatments for R. diversiformis, with the exception of constant dark at 15°C,
where it did not differ significantly in some cases. Although acid scarification
66
67
68
68
68
108